慢性乙型肝炎患者病毒DNA载量与血清HBeAg的关系研究

Relationship between HBV DNA loading and serum HBeAg in patients with chronic hepatitis B

目的探讨慢性乙型肝炎患者乙型肝炎病毒(HBV)DNA载量与血清e抗原(HBeAg)的关系,为临床诊断和治疗慢性乙型肝炎提供实验室依据。方法以2014年4月-2015年10月医院收治239例慢性初治乙型肝炎患者和180例无临床症状且HBV表面抗原(HBsAg)阳性HBV携带者为研究对象;HBV-DNA载量采用实时荧光定量PCR法测定,血清HBeAg水平采用酶联免疫吸附法(ELISA法)测定,分析HBV-DNA载量与血清HBeAg水平的关系。结果 239例慢性初治乙型肝炎患者血清HBeAg阳性162例,阳性率为67.78%;按照血清HBeAg阳性定量检测结果将慢性初治乙型肝炎患者分为4组,HBV-DNA载量Ⅳ组>Ⅲ组>Ⅱ组>Ⅰ组,差异有统计学意义(F=12.083,P<0.05);180例HBV携带者血清HBeAg阳性113例,阳性率为62.78;血清HBeAg阳性患者HBV-DNA载量对数值为6.89±1.24,高于血清HBeAg阴性患者4.56±1.11,差异有统计学意义(t=8.937,P=0.009);按照血清HBeAg阳性定量检测结果将HBV携带者分为4组,HBV-DNA载量Ⅳ组>Ⅲ组>Ⅱ组>Ⅰ组,差异有统计学意义(F=13.986,P<0.05)。结论随着血清HBeAg水平的升高,HBVDNA载量呈逐渐增加的趋势,通过定量测定血清HBeAg水平可评估慢性初治乙型肝炎患者和HBV携带者的HBV-DNA载量。

OBJECTIVE To explore the relationship between hepatitis B virus（HBV）DNA loading and serum e antigen（HBeAg）in patients with chronic hepatitis B so as to provide the laboratory evidence for diagnosis and treatment of chronic hepatitis B.METHODS A total of 239 patients with chronic hepatitis B and 180 HBV carriers with HBsAg positive who treated in hospital from Apr.2014 to Oct.2015 were selected as study objects.HBV-DNA loading were detected by real-time fluorescence quantitative PCR method,and the serum HBeAg levels were detected by enzyme-linked immunosorbent assay（ELISA）,then the relationship between HBV DNA loading and serum HBeAg was analyzed.RESULTS A total of 162 patients with positive serum HBeAg were found in 239 chronic hepatitis B patients,and the positive rate was 67.78%.The patients with chronic hepatitis B virus were divided into four groups according to the results of serum HBeAg positive quantitative detection,the HBV-DNA loading in Ⅳ group〉Ⅲ group〉Ⅱ group〉Ⅰ group,and the difference was significant（F=12.083,P〈0.05）.A total of113 patients were found to have positive serum HBeAg in 180 HBV carriers,and the positive rate was 62.78%.The log value of HBV-DNA in patients with positive serum HBeAg was 6.89±1.24,which was higher than 4.56±1.11 in patients with negative serum HBeAg,and the difference was significant（t=8.937,P=0.009）.The HBV carriers were divided into four groups according to the results of serum HBeAg positive quantitative detection,and the HBV-DNA value inⅣ group〉Ⅲ group〉Ⅱ group〉Ⅰ group.The difference was significant（F=13.986,P〈0.05）.CONCLUSION With the increase of serum HBeAg level,HBV-DNA loading increases gradually,so HBV-DNA loading in patients with chronic hepatitis B and HBV carriers could be assessed by the results of serum HBeAg quantitative detection.