高效液相色谱法测定蒙药制剂浩森布如-13中胡椒碱的含量

HPLC Determination of Piperine in Mongolian Medicine Preparation Hao Sen Bu Ru-13

目的:建立高效液相色谱法测定蒙药制剂浩森布如-13中胡椒碱的含量。方法:采用Diamonsi L C18(250mm×4.6mm,5μm)色谱柱;以甲醇-水(77∶23)为流动相;流速1.0m L·min^(-1);检测波长343nm;柱温为30℃。结果:胡椒碱在4~40μg·m L^(-1)(r=0.9999)范围内线性关系良好;平均回收率为98.19%(n=9),RSD为0.86%。结论:本方法简便准确、稳定可靠,可用于蒙药制剂浩森布如-13的胡椒碱含量测定。

Objective：To establish a method for the determination of piperine in mongolian medicine preparation Haosenburu -13.Methods：A Diamonsi L-C18（250mm×4.6mm, 5μm）column was used with the mobile phase being chromatographic methanol-water（77∶23）, flow rate being 1.0m L·min^（-1）,detecting wavelength being 343 nm, the column temperature being 30℃. Results：The linear response range from 4 ～40μg·m L^（-1）（r =0.9999,n =6）of piperine;the average recoveries（n =9）of the method was 98.19% with RSD 0.86%.Conclusion：The method is simple,accurate,reproducible and stable,and can be used for the quality control of mongolian medicine preparation Hao Sen Bu Ru -13.