栗属植物总苞鞣质含量的测定

Determination on the Content of Tannin in Castanea Mill Involucre

目的测定栗属植物干燥总苞的鞣质含量。方法收集不同产地的栗属植物总苞如板栗壳、茅栗壳、锥栗壳、日本栗壳,根据《中国药典》附录ⅩA磷钼钨酸-干酪素法测定鞣质含量,采用紫外-可见分光光度法,在760nm波长处测定总酚与不被吸附的多酚的吸光度。结果板栗壳和茅栗壳鞣质含量较高,日本栗壳及锥栗壳鞣质含量较低。结论建立的鞣质含量测定方法准确,可为栗属资源总苞利用提供依据。

Objective To determine the tannins content of dry involucre in plants of Castanea Mill. Methods Collecting involucre of four kinds of plants C. mollissima B1. , C. seguinii Dode, C. henryi Rehd. ＆ Wils, C. crenata Sieb. ＆Cuce from different growing area, visible spectrophotometry was used to determine the tannin content. Results C. mollissima shell and C. seguinii Dode shell had high tannin content, and C. crenata Sieb. ＆Cuce and C. henryi Rehd. ＆ Wils. had low tannin content. Conclusion The method of deterring tannin content is accurate. It can provide basis for use chestnut resources involucre.