花姜酮通过抗氧化应激抑制人气道平滑肌细胞的增殖

Zerumbone inhibit human airway smooth muscle cells proliferation by inte-oxidative stress

目的探讨花姜酮对转化生长因子β（transforming growth factor-β，TGF-β）诱导人气道平滑肌细胞（human airway smooth muscle cells，HASMCs）增殖的抑制作用及其机制。方法用CCK-8的方法检测细胞增殖；用荧光显微镜观察2，7-二氯荧光乙酰乙酸（DCFH—DA）染色后细胞内的活性氧（reactive oxygen species，ROS）；用流式细胞术检测细胞内ROS水平。结果TGF-β1（1μg／L）作用72h后可以明显促进HASMCs的增殖（P〈0．05）；花姜酮可以呈浓度依赖性的抑制HASMCs的增殖（P〈0．05），抑制细胞内ROS的水平，以花姜酮组（30mmol／L）的抑制作用最明显（P〈0．01）。结论花姜酮可以抑制HASMCs的增殖，其机制可能是通过抑制细胞内的ROS产生。

Objective To explore whether zerumbone may inhibit human airway smooth muscle cells proliferation and including mechanisms. Methods Cell proIiferation was analyzed by the CCK-8 assay ; Intracellular reactive oxygen species （ROS） was detected with Fluorescence microscope and the flow cytometryin which HASMCs were incubated with an oxidation sensitive fluorescentprobe, 2, 7-dichlorofluorescindiacetate （1）CFH-DA）.Results TGF-β1 （1 μg/L） had an obviously proliferative effect on HASMCs with 72 h （ P 0.05）. Zerumbone inhibited the proliferative effect on HASMCs, there is dose dependent （P 〈 0.05）. Zerumbone inhibited the level of ROS in HASMCs, which was obviously in zerumbone （30 mmol/L）（ P〈 0.01）. Conclusions Zerumbone inhibited the HASMCs proliferation, the mechanisms in which may inhibit the ROS.