NUA-DAS生态滤池脱氮效果与反硝化菌特征研究

Nitrogen Removal and the Characteristics of Denitrification Bacteria Using NUA-DAS Ecofilter

构建小型酸中和残渣(neutralized-used acid residue,NUA)和脱水铝污泥(dewatered alum sludge,DAS)联合生态滤池,研究了NUA-DAS生态滤池的脱氮效果和反硝化菌特征.系统运行稳定后,装置总出水中COD、TN、NO_3^--N的平均去除率达到60%、70%和95%,出水中NO_3^--N的浓度范围只有0.02~0.55 mg·L^(-1).采用PCR-DGGE分子生物学技术检测系统运行30d和60d各滤料层中含3类基因(nirS、nirK和nosZ)的反硝化菌群落特征,包括丰富度及相似度.结果表明,系统运行30 d和60 d里,nirS、nirK和nosZ基因反硝化菌丰富度均有明显增加,且处在各个滤料层中的反硝化菌丰富度基本相同.NUA和DAS滤料中检测出3类基因丰富度指数大小均为nosZ>nirK>nirS.运行时间对反硝化菌的群落结构影响并不明显,但空间位置有一定影响.反硝化菌在NUA中的适应能力优于DAS,3类基因中nirK基因对滤料环境的适应能力最强.

A small-scale combined ecofilter was constructed using neutralized-used acid residue（ NUA） and dewatered alum sludge（ DAS）,and the nitrogen removal for wastewater treatment and characteristics of denitrification bacteria using the NUA-DAS ecofilter were studied. After the system was stabilized,the average removal rates of COD,TN,NO_3^--N in the final effluents were 60%,70%and 95%,respectively,and the range of NO_3^--N concentration in the effluents was only 0. 02-0. 55 mg·L^（-1）. Furthermore,the richness and similarity of three types of functional genes（ nirS,nirK and nosZ） for denitrification in different substrates during the operation period were analyzed using PCR-DGGE method. These results showed that the richness of all denitrification bacteria at NUA and DAS increased remarkably after operation for 30 and 60 days compared to that in fresh substrate,and the richness was basically the same for the same kind of gene at the identical substrate regardless of depth gradient and operation period. The richness of nirS,nirK and nosZ detected in the NUA and DAS followed the order of nosZ〉 nirK 〉nirS. It was also revealed that spatial location had an apparent influence on the community structure of denitrifying bacteria（ nirS,nirK and nosZ） but operation time had no obvious effect.Finally,nirK might be the most suitable for the environment in the system,and the adaptive capacity of denitrification bacteria（ nirS,nirK and nosZ） in NUA could be superior to that in DAS.