新疆昆仑雪菊多糖的分离纯化及其结构的初步鉴定

Separation and purification of polysaccharides from Kunlun snow chrysanthemum in Xinjiang and preliminary identification of its structure

以新疆昆仑雪菊为原料，采用超声波辅助法提取雪菊多糖（KSCP），经脱蛋白、脱色、透析、DEAE一52层析柱和SephadexG-100凝胶层析柱纯化后，获得两个多糖组分（KSCPl和KSCP2）；将分离纯化后的多糖组分经紫外光谱分析法、冻融分析法、SephadexG一100凝胶柱层析法对其纯度进行鉴定；采用凝胶渗透色谱法和气质联用法（GC—MS）对KSCP分子质量范围和单糖组成进行分析。结果表明，KSCPl和KSCP2均为单一组分；KSCPl是由葡萄糖、阿拉伯糖、半乳糖、木糖4种单糖组成，其摩尔比为10．53：5．02：4．96：1，分子质量范围为820048700U；KSCP2主要由葡萄糖、阿拉伯糖、半乳糖3种单糖组成，其摩尔比为1：2．78：5．07，分子质量范围为6100-6500U。

Using Kunlun snow chrysanthemum in Xinjiang as the raw material, snow chrysanthemum polysaccharides （KSCP） were extracted by ultra- sonic-assisted method. The extracts were purified by deproteinization, decoloration, dialysis, DEAE-52 chromatography column and SephadexG-100 gel chromatography column, and two polysaccharide components （KSCP 1 and KSCP2） were obtained. The purity of polysaccharide components were identified by ultraviolet spectrum analysis, freezing and thawing method and SephadexG-100 gel column chromatography. Meanwhile, the range of KSCP molecular mass and monosaccharide composition were analyzed by gel permeation chromatography （GPC） and GC-MS. The results showed that KSCP1 and KSCP2 were both single composition. KSCP1 was composed of four kinds of monosaccharide including glucose, arabinose, galac- tose and xylose, the molar ratio was 10.53：5.02：4.96：1, and the range of molecular mass was 8 200-8 700 u. KSCP2 was composed of three kinds of monosaccharide including glucose, arabinose and galactose, the molar ratio was 1：2.78：5.07, and the range of molecular mass was 6 100-6 500 u.