摘要
以新疆昆仑雪菊为原料,采用超声波辅助法提取雪菊多糖(KSCP),经脱蛋白、脱色、透析、DEAE一52层析柱和SephadexG-100凝胶层析柱纯化后,获得两个多糖组分(KSCPl和KSCP2);将分离纯化后的多糖组分经紫外光谱分析法、冻融分析法、SephadexG一100凝胶柱层析法对其纯度进行鉴定;采用凝胶渗透色谱法和气质联用法(GC—MS)对KSCP分子质量范围和单糖组成进行分析。结果表明,KSCPl和KSCP2均为单一组分;KSCPl是由葡萄糖、阿拉伯糖、半乳糖、木糖4种单糖组成,其摩尔比为10.53:5.02:4.96:1,分子质量范围为820048700U;KSCP2主要由葡萄糖、阿拉伯糖、半乳糖3种单糖组成,其摩尔比为1:2.78:5.07,分子质量范围为6100-6500U。
Using Kunlun snow chrysanthemum in Xinjiang as the raw material, snow chrysanthemum polysaccharides (KSCP) were extracted by ultra- sonic-assisted method. The extracts were purified by deproteinization, decoloration, dialysis, DEAE-52 chromatography column and SephadexG-100 gel chromatography column, and two polysaccharide components (KSCP 1 and KSCP2) were obtained. The purity of polysaccharide components were identified by ultraviolet spectrum analysis, freezing and thawing method and SephadexG-100 gel column chromatography. Meanwhile, the range of KSCP molecular mass and monosaccharide composition were analyzed by gel permeation chromatography (GPC) and GC-MS. The results showed that KSCP1 and KSCP2 were both single composition. KSCP1 was composed of four kinds of monosaccharide including glucose, arabinose, galac- tose and xylose, the molar ratio was 10.53:5.02:4.96:1, and the range of molecular mass was 8 200-8 700 u. KSCP2 was composed of three kinds of monosaccharide including glucose, arabinose and galactose, the molar ratio was 1:2.78:5.07, and the range of molecular mass was 6 100-6 500 u.
出处
《中国酿造》
CAS
北大核心
2016年第7期74-78,共5页
China Brewing
关键词
昆仑雪菊
多糖
纯化
单糖组成
结构
Kunlun snow chrysanthemum
polysaccharides
purification
monosaccharide compositions
structure