醇类对木醋杆菌JMCW-1合成细菌纤维素的影响

Effects of alcohols on the production of bacterial cellulose by Acetobacter xylinus JMCW-1

研究了不同醇类对木醋杆菌JMCW-1的影响。结果表明,不同醇类的添加对发酵液的p H影响不大;添加乙醇、丙三醇、乙二醇的发酵液中残糖含量均远远高于空白对照组,添加聚乙二醇的发酵液中残糖含量随添加量的增加先稍稍降低再升高随后又降低,添加聚乙烯醇的发酵液残糖含量随添加量的增加逐渐降低;添加乙二醇的发酵液OD值降低,添加其他5种醇的发酵液OD值升高;添加乙醇、丙三醇和聚乙烯醇可以提高细菌纤维素的产量,乙醇添加量为3.5%时,细菌纤维素的干质量最高,达到22.6 g/L,为未添加时的7倍;甲醇的添加对细菌纤维素的产量无明显影响。添加聚乙二醇、乙二醇对细菌纤维素的产量有一定的抑制作用。

The effect of different alcohols on Acetobacter xylinus JMCW-1 was studied. The results showed that different alcohols addition had little effect on the pH of fermentation broth. Residual sugar levels in the broth added ethanol, glycerol, ethylene glycol were much higher than that in con- trol group. Residual sugar levels in the broth added polyethylene glycol first lowered and then increased slightly and subsequently reduced. Residual sugar levels in the broth added polyvinyl alcohol gradually decreased. The OD in the broth added glycol decreased, and the OD in the broth added other alcohols increased. Ethanol, glycerol, and polyvinyl alcohol can increase the yield of bacterial cellulose; the dry weight of bacterial cellulose reached the highest 22.6 g/L with ethanol addition 3.5%, which was seven times of the blank group. Methanol had no significant effect on bacterial cellulose production. Polyethylene glycol and ethylene glycol had a certain inhibition on bacterial cellulose production.