LC-MS/MS法测定食用油中的辣椒素和二氢辣椒素

Determination of capsaicin and dihydrocapsaicin in edible oil by LC-MS/MS

建立了用氨基固相萃取小柱净化提取液，液相色谱串联质谱（LC-MS／MS）法测定食用油中辣椒素和二氢辣椒素含量的检测方法。结果表明，辣椒素在0--100ng／mL范围内线性关系良好（R2=0．9986），平均回收率在70．6％-90．5％，相对标准偏差（RSD，n=3）在3．1％～8．9％，检出限为0．03ixg／kg,二氢辣椒素在0～20ng／mL范围内线性关系良好（R2=0．9988），平均回收率在71．5％～100．7％，相对标准偏差（RSD，n=3）在6．O％～13．2％，检出限为0．006p,g／kg。该方法简便快速、准确可靠，是鉴别地沟油和测定食用油中辣椒素类物质的有效方法。

A method was established for the determination of capsaicin and dihydrocapsaicin in edible oil by solid phase extraction and LC-MS/MS. The results indicated that the capsaicin showed good linear relationship in the range of 0-100 ng/ml （R2=0.998 6）, the average recovery was 70.6%-90.5%, and relative standard deviation （RSD, n=3） was 3.1%-8.9%, and the detection limit was 0.03 μg/kg. The dihydrocapsaicin showed good linear relation- ship in the range of 0-20 ng/ml （R2=0.998 8）, the average recovery was 71.5%-100.7%, relative standard deviation （RSD, n=3） was 6.0%-13.2%, and the detection limit was 0.006 Ixg/kg. The method was simple, rapid, accurate and reliable, and it was an effective method for the identification of waste oil and determination of capsaicin in edible oil.