扫描探针显微技术测量血管内皮细胞的弹性

Elasticity measurement of endothelial cells by scanning probe microscopy

血管内皮细胞的功能与许多疾病间存在关联,但现在对其功能好坏的定量描述仍十分少见。该文以内皮细胞的弹性作为衡量其功能的一个标准。通过理论建立压痕测量杨氏模量的计算方法,并利用扫描探针显微镜从实验上得到了正常及过氧化氢处理过的人脐静脉内皮细胞的杨氏模量。发现过氧化氢处理后细胞的杨氏模量升高,表明通过杨氏模量来表征细胞活性是可行的。

It is known that the function of endothelial cells is associated with the presence of many diseases. However, so far the quantitative descriptions of their function have been seldom investigated. In this paper, the function of endothelial cells is evaluated by the elastic modulus. The theoretical calculation for detecting their Young＇s modulus based on indentation theory is established. The Young＇s moduli of the normal endothelial cells and the endothelial cells after hydrogen peroxide treatment are investigated by the scanning probe microscopy. It is found that after hydrogen peroxide treatment the Young＇s modulus of the endothelial cells will be increased. Thus, it is proved that the activity of the cell can be characterized by its elasticity. Finally, errors in the measurement are discussed.