前列腺素E_2对大鼠巨噬细胞株NR8383合成血管内皮生长因子促进人脐静脉血管内皮细胞成管、迁移的影响

Media of rat macrophage NR8383 cells with prostaglandins E_2-induced VEGF overexpression promotes migration and tube formation of human umbilical vein endothelial cells

目的探究前列腺素E_2(PGE_2)对大鼠巨噬细胞株NR8383细胞合成血管内皮生长因子(VEGF)的调控作用以及对人脐静脉内皮细胞(HUVEC)趋化成管的影响。方法分别采用0.1 nmol/L PGE_2、1 nmol/L PGE_2、1 nmol/L PGE_2+10 nmol/L EP2受体抑制剂AH6809+10 nmol/L EP4受体抑制剂AH23848处理的NR8383细胞作为各实验组,选择未经PGE2以及其特异性受体抑制剂处理的NR8383细胞作为对照组,采用Western blot和qPCR方法检测各组NR8383细胞内VEGF蛋白以及m RNA的表达水平;收集以上各处理组的细胞培养上清液分别刺激HUVECs,运用TRANSWELL小室、Matrigel胶细胞成管实验等实验方法,观察PGE_2调控巨噬细胞对HUVEC迁移效应和成管能力的影响。结果随着NR8383细胞培养液中加入PGE_2浓度增高,其VEGF蛋白表达和VEGF mRNA的表达水平显著升高(P<0.05);0.1 nmol/L、1 nmol/L PGE_2处理过的NR8383细胞培养上清液可以显著增加HUVEC细胞形成的小管面积,形成小管面积随着PGE_2处理浓度的增加而增加(P<0.05);HUVECs的迁移运动也随着PGE_2处理浓度的升高不同程度的增强,HUVECs趋化的数量显著升高(P<0.05);研究发现PGE_2特异性的EP2/EP4受体拮抗剂AH6809/AH23848,可以显著抑制PGE_2增强NR8383细胞内VEGF mRNAs表达的作用并且也显著抑制PGE2增强NR8383细胞促进HUVECs成管和趋化能力的效应(P<0.05)。结论 PGE2可以通过作用NR8383细胞表面对应的EP2/EP4受体调控VEGF的合成,促进HUVEC趋化和成管效应。

Objective To investigate the effect of prostaglandins E_2（PGE_2） in enhancing vascular endothelial growth factor（VEGF） expression in a rat macrophage cell line and the effect of the media from PGE_2-inuced rat macrophages on angiogenetic ability of human umbilical vein endothelial cells（HUVECs） in vitro.Methods Western blotting and q PCR were employed to investigate the expressions of VEGF protein and m RNAs in rat macrophage cell line NR8383 stimulated by PGE_2 in the presence or absence of EP_2 receptor inhibitor（AH6809） and EP_4 receptor inhibitor（AH23848）.Conditioned supernatants were obtained from different NR8383 subsets to stimulate HUVECs,and the tube formation ability and migration of the HUVECs were assessed with Transwell assay.Results PGE_2 stimulation significantly enhanced the expression of VEGF protein and m RNAs in NR8383 cells in a dose-dependent manner.The supernatants from NR8383 cells stimulated by PGE_2 significantly enhanced tube formation ability of HUVECs（P〈0.05） and promoted the cell migration.Such effects of PGE_2 were blocked by the application of AH6809 and AH23848.Conclusion PGE_2 can dose-dependently increase VEGF expression in NR8383 cells,and the supernatants derived from PGE2-stimulated NR8383 cells can induce HUVEC migration and accelerate the growth of tube like structures.PGE2 are essential to corpus luteum formation by stimulating macrophages to induce angiogenesis through EP2/EP4.