薏苡仁不同组分对脾虚水湿不化大鼠空肠基底膜相关基因的影响

Effect of different components of Coix Seed on related genes in jejunal basolateral membrane of rats with syndrome of spleen deficiency and water dampness

目的探讨薏苡仁不同组分对脾虚水湿不化大鼠空肠基底膜相关转运蛋白和因子基因的影响。方法复制脾虚水湿不化大鼠模型,以事先提取的薏苡仁不同组分对模型进行干预,对空肠进行基因芯片测定;以Quick-GO对差异基因进行富集,对注释到的基底膜组件进行主成分分析(PCA),挖掘最具有代表性的基因。结果 7组样本共注释到差异基因20条,对注释到的差异基因,以主成分分析进行降维,结果发现当提取主成分数目为3时,可以解释资料89.7%的变异,对数据的解释程度良好。对各主成分作用的载荷和得分进行相关性分析发现,在最具有区分价值的第一主成分(PC1)和第二主成分(PC2)上,薏苡仁各治疗组非常接近,说明它们对基底膜相关蛋白的作用类似;而在PC2和第三主成分(PC3)上,它们仍具有各自的特点。进一步探讨其作用靶点,对PC1、PC2和PC3进行归一化后发现,各基因权重不同,权重前10位的为:溶质载体家族22a7(Slc22a7)、碳酸酐酶(Car9)、血管紧张素II受体1b(Agtr1b)、S100钙结合蛋白G(S100g)、水通道蛋白9(Aqp9)、膜突蛋白(Msn)、水通道蛋白3(Aqp3)、溶质载体家族4a1(Slc4a1)、溶质载体家族4a10(Slc4a10)、耳畸蛋白(Otof)。结论薏苡仁能够修复造模刺激导致的小肠损伤,提高模型溶质载体家族(Slc)表达,使小肠募集白细胞的能力得以恢复,这可能是薏苡仁"健脾"作用的体现。薏苡仁能够降低脾虚水湿不化模型的水通道蛋白3(Aqp3)水平,减少水液从肠腔向机体组织的吸收,是薏苡仁"利湿"作用的体现。

Objective To investigate the effects of different components of Coix Seed（Semen Coicis, Yiyiren）on certain genes of transporters and factors in jejuna basolateral membrane of rat with syndrome of spleendeficiency and wate rdampness.Methods Aftermodeling,theratswithsyndromeofspleen deficiency and water dampness were treated by different components extracted from Coix Seed.Then jejunum was tested by gene chip;functional-annotation-gene to basolateral plasma membrane was analyzed by Quick-GO,and the most representative gene was found out by principal component （PC ）analysis.Results Therewere 20 differential genes in sevengroups.After dimension reduction by principal component analysis （PCA ）,89.7% of variance could be explained when the number of principal component was three.By correlation analysis between loading and score in PC1 and PC2,the expression of genes on basolateral plasma membrane of PC1 was very close to those of PC2,showing the similar function of different components of Coix Seed on jejuna basolateral membrane.However,there was a different situation in PC2 and PC3.Normalization of the weight of PC1,PC2 and PC3 showed that the leading weight genes were Slc22a7,Car9,Agtr1b,S100g,Aqp9,Msn,Aqp3,Slc4a1,and Slc4a10.Conclusion Coix Seed can repair the injury of small intestine due to modeling stimulation, and upregulate SLC expression leading to regain the ability of raising white blood cell,materializing the TCM abstract term “invigorating spleen”.Coix Seed can downregulate AQP3 expression of the model, and reduce liquid absorption from intestinal lumen,materializing the term of“eliminating dampness”.