宁夏枸杞叶中的化学成分

Chemical components from leaves of Lycium barbarum

目的研究中药宁夏枸杞叶中的化学成分。方法使用70%乙醇对宁夏枸杞叶进行提取,所得浸膏混悬后依次用石油醚、乙酸乙酯、正丁醇进行萃取。分别取乙酸乙酯层和正丁醇层,使用硅胶柱色谱、凝胶柱色谱、制备液相色谱及薄层色谱等色谱技术进行分离纯化,利用理化常数和核磁共振、质谱等波谱学技术对分离得到的化合物进行结构鉴定。结果从宁夏枸杞叶70%乙醇提取物乙酸乙酯萃取层和正丁醇萃取层中分离鉴定了10个化合物,分别为对甲氧基苯甲酸(1),对甲氧基肉桂酸(2),东莨菪素(3),对羟基苯甲醛(4),对羟基苯乙酮(5),(Z)-2-亚乙基-3-甲基丁二酸(6),槲皮素(7),腺苷(8),东莨菪苷(9),芦丁(10)。结论化合物1,2,4,6,8为首次从枸杞属植物中分离得到,化合物3,5,9为首次从宁夏枸杞叶中分离得到。

Objective To study the chemical components in the leaves of Lyciu mbarbarum（Barbary Wolfberry,Gouqi）.Methods Aftersuspensionofthecrudeextractfromleavesby70%alcohol,the extraction was performed with petroleum ether,ethyl acetate and n-butanol successively.Then repeated silica gel column chromatography,Sephadex LH-20 chromatography,preparative HPLC and thin layer chromatography were applied for isolation and purification of the compounds in ethyl acetate and n-buta-nol extracts.Chemical structures of the compounds were identified by physical properties and spectra a-nalysisincluding NMRand ESI-MS.Results Atotal10 componentswereidentified：4-methoxybenzoic acid （1）,4-methoxycinnamic acid （2）,scopoletin （3）,4-hydroxybenzaldehyde （4）,4′-hydroxyaceto-phenone （5 ）,（Z）-2-ethylidene-3-methylsuccinic acid （6 ）,quercetin （7 ）,adenosine （8 ）,scopolin （9）,rutin（10）.Conclusion 1,2,4,6,8wereisolatedforthefirsttimefromLyciumL.,and3,5, 9 were isolated from Lycium barbarum L.for the first time.