甲硝唑片两种不同含量测定方法的比较

Comparison of Two Different Metronidazole Tablets Determination Method

目的：建立内标法测定甲硝唑片的含量，内标法与外标法测定结果进行比较，内标法准确度更高。方法：采用高效液相色谱法。色谱柱为Waters symmetry-C18，流动相为甲醇-0.02mol/L草酸溶液（40：60，V/V），检测波长为268 nm，流速为1.0 ml/min，柱温为25℃，进样量为20μl。结果：甲硝唑质量浓度在10.0～150.0μg/ml范围内与峰面积呈良好线性关系（r=0.9997），平均加样回收率为99.53%，RSD=0.81%（n=3）。结论：本法简便、准确，专属性好，精密度高，可用于甲硝唑片的质量控制。

Objective：Metronidazole tablets, the internal standard method and external standard assay results were compared, the internal standard method to establish the accuracy of the determination within higher standard method. Method： Waters symme-try-C18 , Methanol-0.02mol/L oxalic acid solution as the mobile phase （40：60：1,V/V）.The detection wavelength was 268nm, The flow rate was 1.0 ml/min, the column temperature was 25℃, the injection volume was 20μl. Result：The linear range of metronida-zole was 30~110.0μg·ml-1 （r=0.9997）. The average recovery was 99.53%and RSD was 0.81%（n=9）. Conclusion：The method is sim-ple, accurate and suitable. It can be used for quality control of metronidazole tablets.