摘要
Cherry virus A（CVA） is a member of the genusCapilovirus, in the familyBetalfexiviridae. The infection rate of CVA was high in sweet cherry in China. We determined the complete nucleotide sequences of two isolates of CVA from Tai’an, Shan-dong Province, China using high ifdelity PCR enzymes and speciifc primer pairs for amplifying long fragments in RT-PCR and RACE. The ful-length sequences from isolates ChTA11 and ChTA12 are both 7382 nucleotide （nt） long, excluding the poly（A） tail, encode two open reading frames （ORFs） and have similar genome organization to the two isolates in Gen-Bank. The complete nucleotide sequence of ChTA11 is 98.2 and 81.2% nt identity to the isolates from Germany and India in GenBank, respectively, and the ChTA12 isolate is 98.2 and 81.0% similar. Analysis of the nucleotide and amino acid sequences showed that the domain of unknown function （DUF1717） is more variable compared with other domains. This is the ifrst report of the complete nucleotide sequences of CVA isolates infecting sweet cherry in China.
Cherry virus A（CVA） is a member of the genusCapilovirus, in the familyBetalfexiviridae. The infection rate of CVA was high in sweet cherry in China. We determined the complete nucleotide sequences of two isolates of CVA from Tai’an, Shan-dong Province, China using high ifdelity PCR enzymes and speciifc primer pairs for amplifying long fragments in RT-PCR and RACE. The ful-length sequences from isolates ChTA11 and ChTA12 are both 7382 nucleotide （nt） long, excluding the poly（A） tail, encode two open reading frames （ORFs） and have similar genome organization to the two isolates in Gen-Bank. The complete nucleotide sequence of ChTA11 is 98.2 and 81.2% nt identity to the isolates from Germany and India in GenBank, respectively, and the ChTA12 isolate is 98.2 and 81.0% similar. Analysis of the nucleotide and amino acid sequences showed that the domain of unknown function （DUF1717） is more variable compared with other domains. This is the ifrst report of the complete nucleotide sequences of CVA isolates infecting sweet cherry in China.
基金
supported by the Special Fund for Agro-scientific Research in the Public Interest, China (201203076)
the National Natural Science Foundation of China (31471752)
