人脑胶质瘤细胞体外多柔比星敏感性实验研究

Invitro chemotherapy sensitivity experiment with adriamycin in patients of brain gliomas

目的:探讨人脑胶质瘤体外培养后对多柔比星的敏感性,以期指导多柔比星对脑胶质瘤的间质化疗。方法:对脑胶质瘤手术后的组织进行原代细胞培养和纯化,采用MTT法进行多柔比星等多种化疗药物体外药敏试验。结果:对44例人脑胶质瘤手术标本进行体外培养及药敏试验,获得药敏结果 38例,其体外药敏成功率86.4%。单用和联用多柔比星均对原代培养的脑胶质瘤细胞有较好的抑制作用,脑胶质瘤体外培养细胞对多柔比星单用或联合使用都有较高的敏感率,多柔比星化疗对复发脑胶质瘤细胞的敏感率较原发胶质瘤高。结论:MTT法体外药敏试验显示多柔比星对脑胶质瘤细胞有较好的抑制作用,并有较高的敏感率,适合用于脑胶质瘤的间质化疗。

Objective： To investigate the sensitivity of adriamycin to the invitro-cuhured brain glioma in order to guide the adriamycin interstitial chemotherapy for brain glioma. Methods： To conduct primary cell culture and purification with glioma specimens after surgery, then MTT assay was utilized to have invitro drug sensitivity tests with such multi-chemotherapeutic drugs as adriamycin in brain glioma. Results： Invitro culure and drug sensitivity tests were performed in 44 brain gliomas specimens and succeeded in 38 cases with a success rate of 86.4%. It showed that there was a good inhibitory effect on the invitro-cultured cells of glioma with adriamycin alone or combined chemotherapies. And for recurrent glioma cells, the chemotherapy sensitive rate was higher than that of the primary glioma. Conclusion： The invitro sensitivity test by MTT method shows that adriamycin has not only a good inhibitory effect on the glioma cells but a high sensitive rate, it is suitable for the interstitial chemotherapy for brain gliomas.