高迁移率蛋白A2在睾丸肿瘤组织中的表达及临床意义

Up- regulated expression of HMGA2 in testicular cancer tissue and its clinical significance

目的:探讨HMGA2在睾丸癌组织中的表达及意义。方法:运用荧光定量PCR(Q-PCR)和Western blot方法检测HMGA2mRNA和蛋白在17例睾丸肿瘤和15例正常睾丸组织中的表达差异、免疫组织化学(IHC)方法检测HMGA2蛋白在44例睾丸肿瘤,30例良性组织芯片中的表达差异。结果:HMGA2mRNA表达在癌组织中明显高于良性组织:(9.09±2.95)vs.(3.86±1.66),P=0.036、HMGA2蛋白在癌肿组织中的表达亦显著高于良性组织:(9.27±2.91)vs.(4.66±1.62),P=0.037。HMGA2蛋白的表达高低和睾丸肿瘤的临床分期及肿瘤大小密切相关,P<0.05。结论:HMGA2在睾丸肿瘤组织中上调表达并和肿瘤进展相关,有望成为睾丸肿瘤治疗的分子靶点。

Objectives： To investigate the expression and significance of HMGA2 in testicular cancer tissue. Methods： Using the fluorescent quantitative PCR（ Q- PCR） and Western blot methods to detect HMGA2 mRNA and protein in 17 cases of testicular tumors and 15 cases of normal testicular tissue,immunohistochemical（ IHC） method to detect HMGA2 protein in 44 cases of testicular tumors and 30 cases of benign tissue by microarray. Results： HMGA2 mRNA expression in cancerous tissue was significantly higher than that of benign group（ 9. 09 ± 2. 95 VS 3. 86 ± 1. 66,P = 0. 036）; HMGA2 protein expression in tumor tissue was significantly higherthan that of benign organization（ 9. 27 ± 2. 91 VS 4. 66 ± 1. 62,P = 0. 037）. HMGA2 protein expression levels in testicular tumors were associated with clinical staging and the tumor size（ P〈0. 05）. Conclusion： Up- expressed of HMGA2 in testicular cancer is associated with tumor progression and size,which is expected to become the molecular targets of testicular tumor treatment.