胃癌组织中Vav3表达与肿瘤侵袭转移的关系及临床意义研究

Relation Between Vav3 Expression and Tumor Invasion and Metastasis in Gastric Cancer Tissue and the Clinical Significance

目的探讨胃癌组织中Vav3表达与肿瘤侵袭转移的关系及其临床意义。方法选取2014年5月—2015年5月于河北医科大学第四医院外三科住院并行肿瘤切除的胃癌患者92例。收集患者胃癌组织及癌旁正常组织（距肿瘤边缘〉3 cm,镜检无癌细胞）的石蜡标本,采用免疫组化法检测Vav3、Rel A、增殖细胞核抗原（PCNA）、基质金属蛋白酶2（MMP-2）在胃癌组织及癌旁正常组织中的表达;分析胃癌组织中Vav3表达强度与临床病理特征的关系;Vav3、Rel A、PCNA、MMP-2表达之间的关系采用Spearman秩相关分析。结果胃癌组织中Vav3的阳性表达率为78.3%（72/92）,高于癌旁正常组织的40.2%（37/92）（χ^2=27.572,P〈0.001）;胃癌组织中Rel A的阳性表达率为88.0%（81/92）,高于癌旁正常组织的52.2%（48/92）（χ^2=28.242,P〈0.001）;胃癌组织中PCNA的阳性表达率为73.9%（68/92）,高于癌旁正常组织的47.8%（44/92）（χ^2=13.143,P〈0.001）;胃癌组中MMP-2的阳性表达率为69.6%（64/92）,高于癌旁正常组织的48.9%（45/92）（χ^2=8.125,P=0.004）。Vav3强表达患者性别、年龄、浸润深度、远处转移情况与Vav3弱表达比较,差异无统计学意义（P〉0.05）;Vav3强表达患者肿瘤分化程度、肿瘤直径、淋巴结转移程度与Vav3弱表达比较,差异有统计学意义（P〈0.05）。Vav3表达与Rel A、PCNA、MMP-2表达均呈正相关（r_s=0.338,P〈0.001;r_s=0.225,P=0.031;r_s=0.267,P=0.010）;Rel A表达与PCNA表达呈正相关（r_s=0.336,P=0.001）;Rel A表达与MMP-2表达无直线相关关系（r_s=0.176,P=0.094）;PCNA表达与MMP-2表达无直线相关关系（r_s=0.188,P=0.072）。结论胃癌组织中Vav3存在过表达现象,Vav3表达增强与肿瘤转移及进展有关。Vav3可能通过调节Rel A、PCNA、MMP-2表达而参与胃癌侵袭转移。

Objective To investigate the relation between Vav3 expression and tumor invasion and metastasis in gastric cancer tissue and the clinical significance. Methods We collected the paraffin specimens of gastric cancer tissue and carcinoma side normal tissue from 92 patients who received resection for gastric carcinoma in the Third Surgery Department of the Fourth Affiliated Hospital of Hebei Medical Univer_sity from May 2014 to May 2015. The expressions of Vav3,Rel A,PCNA and MMP-2 in gastric cancer tissue and carcinoma side normal tissue were detected by immunohistochemistry staining. The relation between Vav3 expression and clinical and pathological characteristics in gastric cancer tissue was analyzed. The relation among Vav3,Rel A,PCNA and MMP-2 expression was investigated by Spearman correlation analysis. Results The positive expression rate of Vav3 in gastric cancer tissue was 78. 3%（ 72 /92）, higher than 40. 2%（ 37 /92） in carcinoma side normal tissue（ χ~2= 27. 572,P〈0. 001）. The positive expression rate of Rel A in gastric cancer tissue was 88. 0%（ 81 /92）, higher than52. 2%（ 48 /92） in carcinoma side normal tissue（ χ~2= 28. 242,P〈0. 001）. The positive expression rate of PCNA in gastric cancer tissue was 73. 9%（ 68 /92）,higher than 47. 8%（ 44 /92） in carcinoma side normal tissue（ χ~2= 13. 143,P〈0. 001）. The positive expression rate of MMP-2 in gastric cancer tissue was 69. 6%（ 64 /92）, higher than 48. 9%（ 45 /92） in carcinoma side normal tissue（ χ~2= 8. 125, P = 0. 004）. There was no significant difference in gender, age, depth of infiltration,and distant metastasis between high expression of Vav3 and low expression of Vav3（ P〈0. 05）. There was significant difference in tumor differentiation degree, tumor diameter and the extent of lymph node metastasis between high expression of Vav3 and low expression of Vav3（ P〈0. 05）. The expression of Vav3 had positive correlation with the expression of Rel A,PCNA and MMP-2（ r_s= 0. 338,P〈0. 001; r_s= 0. 225,P = 0. 031; r_s= 0. 267,P = 0. 010）. The expression of Rel A had positive correlation with the expression of PCNA（ r_s= 0. 336,P = 0. 001）; the expression of Rel A had no linear correlation with the expression of MMP-2（ r_s= 0. 176,P = 0. 094）. The expression of PCNA had no linear correlation with the expression of MMP-2（ r_s= 0. 188,P = 0. 072）. Conclusion There is overexpression of Vav3 in gastric cancer tissue,and the increase of Vav3 is associated with tumor metastasis and progress. Vav3 is involved in the progress and metastasis of gastric cancer by regulating Rel A,PCNA and MMP-2 expression.