致死剂量微波辐照后小鼠肝超微结构及HSP70表达的变化

Ultrastructural changes and HSP70 expression in mice liver after microwave irradiation with lethal dose

目的观察致死剂量微波辐照后小鼠肝超微结构及热休克蛋白70(HSP70)表达的变化,探讨其在法医学鉴定中的意义。方法将昆明种小鼠分为正常对照组和辐照组,辐照组小鼠采用峰值功率密度为129 W/cm^2的微波全身一次辐照30 min致小鼠死亡后,立即用透射电镜观察小鼠肝的超微结构变化;采用反转录-聚合酶链反应(RT-PCR)法检测小鼠肝HSP70 mRNA表达变化,免疫印迹法检测小鼠肝HSP70蛋白表达变化。结果致死剂量微波辐照后小鼠肝细胞胞浆点片状溶解,线粒体嵴和膜溶解消失;HSP70 mRNA和蛋白水平表达显著上调,与正常组对照比较,差异具有极显著统计学意义(P<0.01)。结论微波辐照致死可导致小鼠肝细胞胞浆溶解、线粒体损伤、HSP70 mRNA和蛋白水平表达明显上调,可作为微波辐照致死鉴定的重要参考指标。

Objective To investigate the uhrastructural changes and HSPT0 expression in liver of mice after microwave irradiation with lethal dose and to explore the application of these indexes as the basis of medical identification in microwave irradiation induced death. Methods The mice were divided into the control group and the irradiation group. Mice of the irradiation group were induced death by whole body exposure to 129 W/cm2 microwave irradiation for 30 minutes. The ultrastructure of liver was observed by transnlission electron micro- scope;changes of the HSP70 mRNA and protein expression in liver were detected by reverse transcription polymerase chain reaction （ RT- PCR） and Western blotting respectively. Results Liver cytoplasm was observed dissolved with points and sheets and there were mitochondri- al crest and membrane solution in the irradiation group. And the HSP70 mRNA and protein expression level increases significantly compared with the control group with statistically significant difference （ P 〈 0.01 ）. Conclusion Death induced by microwave irradiation could lead to liver cytoplasm dissolution, mitochondria damage, mRNA and protein expression of HSP70 up-regulation, which may be used as important diagnostic indicators of microwave irradiation induced death.