摘要
以静电纺丝法制得的壳聚糖/聚乙烯醇纳米纤维膜为载体,固定辣根过氧化物酶（HRP）,研究其催化去除水中2,4-二氯苯酚（2,4-DCP）的效果及其影响因素;并与自由酶催化反应作对比研究,分析H2O2添加量、pH值、温度对催化反应的影响;对固定化HRP催化去除2,4-DCP进行了动力学分析,考察了固定化HRP的重复使用性。结果表明：CS/PVA固定化HRP去除2,4-DCP的最佳条件为：H2O2100mmol/L,p H7,35℃,在60min时,去除率达99%。动力学分析表明,其反应符合一级动力学方程,连续使用7次后,固定化HRP对2,4-DCP的去除率为38%。
Horseradish peroxidase（HRP） was immobilised on Chitosan/PVA nanofiberous membrane made by electrostatic spinning method for the removal of 2,4-dichlorophenol（2,4-DCP） in aqueous solution. The effects of several factors such as H_2O_2 concentration, p H value, temperature on 2,4-DCP removal were discussed in detail in comparison with those of free HRP. The degradation kinetics of 2,4-DCP catalysed by immobilised HRP and reusability of immobilised HRP were also investigated. The results indicated that the removal efficiency of 2,4-DCP could reach 99% in 60 minutes when the optimised H_2O_2 concentration and p H value were 100 mmol/L and 7, respectively, at the temperature of 35°C. The kinetic analysis on the removal of 2,4-DCP by immobilised HRP showed that the reaction fitted well with pseudo-first-order reaction kinetic equation. After seven successive uses, the removal rate could keep at 38%.
出处
《上海环境科学》
CAS
2016年第3期126-131,共6页
Shanghai Environmental Sciences
