丹参当归不同配比抗氧化能力分析

Research on Compatibility of Salviae Miltiorrhizae Radix et Rhizoma and Angelicae Sinensis Radix by Anti-oxidation Effects in Vitro

目的:初步探讨丹参,当归及不同配比提取物的体外抗氧化作用。方法:采用分光光度法分别测定丹参、当归及不同比例配比物的还原性,以及对DPPH自由基、超氧阴离子自由基、羟基自由基的清除作用。结果:分别得到不同浓度丹参、当归及配比提取物的总还原性和对DPPH自由基、超氧阴离子、羟自由基抑制率。结论:丹参、当归配比后体外抗氧化能力优于单味药材,最佳配比为1:3。

Objective： To discuss the anti- oxidation effects in vitro of Salviae miltiorrhizae Radix et Rhizoma,Angelicae Sinensis Radix and different compatibility proportion extracts of the two. Methods： The UV spectrophotometry was used to determine the anti- oxidation effects of the extracts of Salviae miltiorrhizae Radix et Rhizoma,Angelicae sinensis Radix and different compatibility proportion extracts of the two. The anti- oxidation effects in vitro were valuated by different assays such as reducing power,DPPH radical scavenging assay,superoxide radical scavenging assay and hydroxyl radicals assay. Results： We obtained the ability of reducing power,the inhibition of DPPH radical,superoxide radical and hydroxyl radicals respectively. Conclusion： Anti- oxidation effects were enhanced after the compatibility. The optimal compatibility proportion of Salviae miltiorrhizae Radix et Rhizoma and Angelicae sinensis Radix for the anti- oxidation effects in vitro is 1： 3.