盐炙对知母中新芒果苷体内药代动力学的影响

Evaluation of Salt Processing on Pharmacokinetics of Neomangiferin in Anemarrhena Rhizoma

目的:建立测定大鼠血浆中新芒果苷HPLC方法,探究盐炙对知母中新芒果苷药代动力学的影响。方法:色谱柱:Ecosil C_(18)柱(250 mm×4.6 mm,5μm);流动相:水-乙腈(15:85);流速:1 m L/min;检测波长:258 nm;柱温:30℃。结果:新芒果苷在生品和盐炙品组大鼠体内药代动力学参数分别为:AUC_(0-t)为(43.913±0.636)、(71.565±1.950)mg/L*h;AUC_(0-∞)为(44.127±0.673)、(71.805±1.926)mg/L*h;C_(max)为(4.539±0.048)、(5.805±0.119)mg/L;MRT_(0-t)为(8.124±0.246)、(8.650±0.398)h;MRT_(0-∞)为(8.290±0.321)、(8.761±0.435)h;t_(1/2)为(4.315±0.472)、(3.989±0.301)h。结论:知母盐炙后促进新芒果苷的吸收,并在一定程度上增加了生物利用度。

Objective： To establish an HPLC method to determine neomangiferin in rat plasma and investigate the effect of salt processing on pharmacokinetics of neomangiferin in Anemarrhena Rhizoma. Methods： It was obtained with Ecosil column（ 250 mm× 4. 6 mm,5 μm） and eluted with acetonitrile and water（ 15： 85） and its flow rate was 1 m L/min. The detection wavelength was set at 258 nm and the column temprature 30 ℃. Results： The main pharmacokinetic parameters neomangiferin in raw and salt products： AUC_（ 0- t）were（ 43. 913 ± 0. 636）（ mg/L＊ h） and 71. 565 ± 1. 950（ mg/L＊ h）; AUC_（ 0- ∞）were（ 44. 127 ± 0. 673）（ mg/L＊ h） and 71. 805 ± 1. 926（ mg/L＊ h）; C_（max）were（ 4. 539 ± 0. 048）（ mg/L） and 5. 805 ± 0. 119（ mg/L）; MRT_（ 0- t）were（ 8. 124 ± 0. 246） h and（ 8. 650 ± 0. 398） h; MRT_（ 0- ∞）were（ 8. 290 ± 0. 321） h and（ 8. 761 ± 0. 435） h; t_（1/2）were（ 4. 315 ±0. 472） h and（ 3. 989 ± 0. 301） h. Conclusion： Salt processing can obviously enhance the absorption of neomangiferin in Anemarrhena Rhizoma and increase the bioavailability.