与亚洲棉光籽突变体DPL972中光籽基因紧密连锁的SSR分子标记的开发与应用（英文）

Simple Sequence Repeat Markers Closely Linked with a New Fuzzless Gene in Fuzzless Mutant DPL972(Gossypium arboreum)

对棉纤维相关性状基因的克隆及遗传分析可为阐明纤维形成机制奠定良好基础。以野生型材料DPL971及其光籽突变体DPL972为亲本构建F2群体,其中光籽与毛籽性状分离比符合3∶1,表明突变体DPL972中的光籽基因为显性单基因(将其命名为Ga Fzl)。结合棉花基因组数据,合成1567对SSR(Simple sequence repeat)引物,覆盖了A组13条染色体,标记多态性分析和群体连锁分析发现在染色体A08上存在15对与光籽基因Ga Fzl连锁的标记,其中最近的标记为SSR82,遗传距离为6.6 c M。本实验完成了Ga Fzl基因的染色体初步定位,开发了15个与其连锁的SSR标记,为下一步图位克隆奠定了基础。

The genetic analysis and cloning of genes associated with cotton fiber characteristics will not only reveal the mechanism of fiber development, but will also aid molecular breeding. The cotton（Gossypium arboretum） mutant DPL972 is fuzzless,providing a crucial germplasm resource for cotton research. An F2 population derived from a cross between the wild-type DPL971 and the mutant DPL972 was constructed, and the results of trait investigation fitted a segregation ratio of 3 ∶1, which indicated that the fuzzless trait was controlled by a single dominant gene（named here as Ga Fzl）. A linkage map was constructed,which included 15 simple sequence repeat markers close to the candidate target gene. The fuzzless gene Ga Fzl was inferred to be located on chromosome A08 and was tightly linked with marker SSR82 at a genetic interval of 6.6 c M.