摘要
探讨IGF-2及IGF-1R蛋白质在子宫蓄脓的表达及与子宫蓄脓发病的相关性。采用免疫组织化学PV法与Western blotting检测5例正常子宫内膜(对照组)、19例子宫蓄脓(试验组)子宫内膜组织中IGF-2及IGF-1R的表达情况。结果表明,免疫组化与Western blotting检测结果一致,IGF-2和IGF-1R在蓄脓子宫组中的表达量极显著高于正常子宫(P<0.01)。对子宫蓄脓组中IGF-1R与IGF-2进行相关性分析,免疫组化结果表明IGF-1R与IGF-2之间低度相关(r=0.423),Western blotting结果显示IGF-1R与IGF-2之间高度相关(r=0.927)。结果提示,IGF-2阳性表达上调及IGF-1R的异常高表达,在子宫蓄脓的发生和发展过程中可能具有重要作用。
To investigate the expression of IGF-2 and IGF-1R protein in endometria of canine pyometra and analyze the possible correlation with the incidence of canine pyometra.Immunohistochemistry PV method and Western blotting were used to measure the IGF-2 and IGF-1R expression characteristic of 5 normal endometria and 19 endometria of canine pyometra.The results showed that the IGF-2 level of endometria from pyometra group was extremely significant higher than that of control group by immunohistochemistry and Western blotting （P＜0.01）.The positive expression rate of IGF-1R in endometria of canine pyometra compared with that in the normal endometria was extremely significant higher by immunohistochemistry and Western blotting （P＜ 0.01）.The correlation analysis of IGF-2 and IGF-1R in canine pyometra showed that the result of immunohistochemistry was low correlation （r=0.423）,the result of Western blotting was high correlation （r=0.927）.The data suggested that increased expression of IGF-2 and IGF-1R played an important role in occurrence of canine pyometra.
出处
《中国畜牧兽医》
CAS
北大核心
2016年第5期1263-1269,共7页
China Animal Husbandry & Veterinary Medicine
基金
天津市财政专项项目<转基因家畜新品种培育
健康养殖新技术研究与开发利用>(2011CZZX)
