白子菜提取物对急性痛风性关节炎大鼠模型抗炎作用的研究

Anti-inflammatory Effect of Gynura divaricata(Linn.) DC. Extract on Rat Models with Acute Gouty Arthriti

目的探讨白子菜提取物对急性痛风性关节炎大鼠模型抗炎作用。方法将72只Wistar大鼠随机分为9组(每组8只):空白组、模型组、秋水仙碱组、白子菜水提取物低、中、高剂量组、白子菜醇提取物低、中、高剂量组。造模5 h后开始连续灌药,7 d后取材,观察各组大鼠关节炎症情况,关节组织单位面积内炎症细胞计数及检测血清白介素-1β(interleukin-1β,IL-1β)、肿瘤坏死因子-α(tumour necrosis factor-α,TNF-α)浓度。结果中、高剂量白子菜提取物组与秋水仙碱组在足趾炎症情况显著低于模型组(P<0.05)。在淋巴细胞、中性粒细胞计数方面各药物组显著低于模型组(P<0.05),各药物组间无明显差异(P>0.05)。秋水仙碱组TNF-α表达水平显著低于模型组(P<0.05)。秋水仙碱组及白子菜醇提取物中剂量组IL-1β表达水平显著低于模型组(P<0.05)。结论秋水仙碱及中剂量白子菜醇提物对急性痛风性关节大鼠模型具有抗炎免疫作用。

Objective To explore the anti-inflammatory of Gynura divaricata （Linn.） DC. extract on rat models with acute gouty arthritis. Methods Seventy-two Wistar rats were randomly divided into nine groups （eight rats in each group）：blank group, model group, colchicines group, ethanol extract of G. divaricata groups （high-dose group, middle-dose group and low-dose group）, water extract of G. divaricata Groups （high-dose group, middle-dose group, low-dose group）. After 5 h of modeling, the rats were given medicine continously. After 7 d of treatment, the inflammatory cells among joint tissue and the concentrations of IL-1β, TNF-α from serum were observed and determined. Results The foot inflammation condition of G. divaricata extract groups （high-dose and middle-dose） and colchicines group were significantly lower than that of model group （P〈0.05）. The lymphocytes, neutrophils among joint tissue in the medicine intervention groups were significantly lower than that of model group （P〈0.05） and there were no significantly difference between each of the two medicine intervention groups （P〉0.05）. The expression of TNF-α in the colchicines group was significantly lower than that in the model group （P〈0.05）. The expression of IL-1β in G. divaricata ethanol-extract groups and colchicines group were significantly lower than that of model group （P〈0.05）. Conclusion Colchicines and G. divaricata ethanol-extract group of middle-dose show significant anti-inflammatory effect on rat models with acute gouty arthritis.