磷酸改性花生壳固定化α-淀粉酶研究

Modified Peanut Shells on α- Amylase Immobilized by Phosphoric Acid

以磷酸改性花生壳为载体固定α-淀粉酶,研究改性后的花生壳吸附固定α-淀粉酶的最优固定化条件以及固定化酶的酶学性质。试验结果表明:用磷酸溶液对粉碎的花生壳颗粒进行浸泡处理来改性,研究出酶固定化最优条件是:酶液/载体比11∶1(m L/mg),缓冲液p H6.0,固定时间8 h和温度35℃。经3次平行试验,所得实际固定化酶活力平均值为27 980 U/g。对游离酶和固定化酶部分酶学性质比较,得出改性固定化后酶的最适反应p H、温度有所改变,为p H=6.0,温度45℃,其储存时间、操作稳定性和耐热性比游离酶更好。

A modified peanut shells asα-amylase immobilized on the enzymatic properties of the immobilized enzyme was detected, study the modified peanut shells fixedα-amylase adsorption optimal immobilization con-ditions and immobilized enzyme enzymatic properties. The results showed that： enzyme immobilization devel oped optimal conditions were：enzyme/carrier 11∶1（mL/mg） buffer pH6.0, 8 h fixed time and Temperature 35℃. After three parallel experiments, the resulting immobilized enzyme actual average of 27 980 U/g. The free enzyme and immobilized enzymatic properties comparison, the immobilized enzyme modified optimum pH, temperature changes, as pH=6.0, temperature was 45℃. At the same storage time, number of operations and the heat resistance was more than the free enzy.