超声波辅助法和乙醇回流法提取葛根素

Extracting Puerarin with Ultrasonic Assisted and Alcohol Refluxing Method

用乙醇做溶剂,分别采用超声辅助法和回流法从葛根中提取有效成分葛根素,考察了反应时间、反应温度、固液比、乙醇浓度等因素对提取率的影响,并采用正交设计进行了优化。研究结果表明,乙醇回流法提取葛根素的最佳工艺条件为:乙醇浓度75%,提取温度90℃,提取时间90 min,固液比为1∶20(g/m L),葛根素的提取率为86.20%;超声波辅助法提取葛根素的最佳工艺条件为:乙醇浓度60%,提取温度80℃,固液比为1∶20(g/m L),提取时间50 min,葛根素的提取率为95.85%。超声波辅助提取的提取率明显高于乙醇回流法。

Active ingredients puerarin was extracted from pueraria via ultrasound method and reflux method, respectively. Effects of reaction time, reaction temperature, solid-liquid ratio, ethanol concentration on the extraction rate were investigated. And then optimized process was designed through orthogonal design. The results demonstrated that the optimum conditions by ethanol extraction was ethanol concentration 75 %, extraction temperature 90℃, extraction time 90 min, solid-iquid of 1∶20 （g/mL）. The resulting extracting rate of puer arin was 86.20%. The optimum conditions of ultrasonic assisted extraction method was ethanol concentration 60%, extraction temperature 80℃, solid-liquid ratio of 1∶20 （g/mL）, extraction time 50 min. The puerarin extraction rate was up to 95.85%. The extraction rate by ultrasonic way was significantly higher than the way of ethanol refluxing.