从尖尾东鳖甲中提取甲壳素的研究

Study on the Extracting Chitin from Anatolica mucronata Reitter

通过单因素试验和正交试验探讨从尖尾东鳖甲(Anatolica mucronata Reitter,1889)中提取甲壳素的最佳条件,并对甲壳素样品进行定性和定量分析。试验结果表明:利用酸浸碱煮法提取甲壳素的最佳试验条件为:氢氧化钠浓度7%、温度87℃、时间5 h、固液比1∶13(g/m L),柠檬酸浓度1.6 g/L、室温、时间28 h、固液比1∶9(g/m L);对在最佳试验条件下提取得到的甲壳素样品进行红外光谱检测,并计算其得率为21.45%。用紫外吸收法和干法灰化法分别测定提取物中残余蛋白质和无机盐含量。

This article discussed the optimum conditions of extracting chitin from Anatolica mucronata Reitter by single-factor and orthogonal test. The chitin sample was carried on the qualitative and quantitative analysis. Test results showed that the optimum conditions by using acid-alkali method to extract chitin were as follows：the NaOH concentration 7%, the temperature 87℃, the time 5 h, the solid-liquid ratio 1∶13 （g/mL）, the citric acid concentration 1.6 g/L, the time 28 h, the solid-liquid ratio 1∶9（g/mL）, at room temperature. Using infrared spectrum to analysis chitin sample extracted under optimum conditions and the extraction rate of chitin was 21.45%. Then residual protein and inorganic salt content in the extract were measured by UV absorption and dry ashing method.