蕤核多糖脱色素工艺的优化研究

Study on Optimization of Prinsepia uniflora Batal Polysaccharide Depigmentation Process

对蕤核多糖脱色素工艺进行优化研究。通过均匀设计试验法U5(54),对多糖溶液p H、树脂添加量、吸附时间、震荡转速4个因素进行了研究,以脱色率和多糖回收率为衡量指标,得到最佳的提取工艺。研究结果表明最佳提取工艺参数为,多糖溶液p H=4.0、树脂添加量4 g、吸附时间4 h、振荡频率100 r/min,在此条件下,蕤核果实多糖溶液的脱色率和多糖回收率可达82.7%、80.2%。

The polysaccharide of Prinsepia uniflora Batal depigmentation process optimization was researched. The uniform experimental design method U5（54）, 4 factors on the polysaccharide solution pH, adding amount of resin, adsorption time, shock speed was studied. The decolorization rate and the recovery rate of polysaccha-rides were measured ,the best extraction process was achieved. The research results showed that the optimum extraction process parameters were as follow：the polysaccharide solution with pH of 4, the shock speed 90 r/min, the adsorption time 5 h, the resin addition amount of 5 g . Under these conditions, decolorization of Prinsepia uniflora Batal fruit polysaccharide solution was up to 82.7%, 80.2%rate of recovery of polysaccharide.