响应面法优化葡萄籽蛋白质提取工艺

Optimizing Extraction Technology of Protein from Grape Seed with Respones Surface Methodology

为了提高赤霞珠葡萄籽的有效利用率,采用碱溶酸沉法提取葡萄籽中的蛋白质,并通过响应面法优化提取条件。在单因素试验的基础上,依据Central Composite中心组合设计原理,采用Design-Expert.8.05数据分析软件,对蛋白质提取进行四因素三水平的优化分析。确立最佳提取条件:NaOH浓度0.001 mol/L,提取温度45℃,提取时间40 min,料液比1∶25(g/mL),在此条件下蛋白质的提取理论值达到9.75 mg/g。

Alkali-solution and acid-isolation method was used to extract protein from Cabernet Sauvignon grape seed to improve the utilization rate of grape seed, in which respones surface analysis was used to optimize extraction conditions. Based on the single factor experiments, the Central Composite design principles and De-sign-Expert.8.05 data ananlysis software were used to select four parameters of response surface design to opti-mize extraction process. The result showed the optimize extraction conditions： the concentration of NaOH 0.001 mol/L, extraction temperature 45℃,extraction time 40 min, liquid to solid ratio 1∶25 （g/mL）, the ex-traction value was 9.75 mg/g.