紫娟茶中花青素提取物含量的不确定度评定

Evaluation for Measurement Uncertainty of Anthocyanins from Zi Juan Tea

紫娟茶由含酸甲醇提取后,经过乙醚、氯仿的液液分配以及Amberlite XAD-7HP树脂的吸附洗脱,得到花青素粗提物。针对花青素提取物,采用DPPH法研究探讨了其抗氧化性及清除自由基的能力,并针对抗氧化性试验进行了不确定度的评定,得到的扩展不确定度为0.132 2(以Trolox mg/kg计),说明测量结果有较高的质量,使用这种抗氧化方法得到的结果是可信的。本研究的结果为紫娟茶花青素的深入研究与有效开发利用,提供了必备的表征条件和理论参考,并有助于食品原料的深度加工和功能性检测方法的应用与开发。

Anthocyanins from Zi juan tea were exteacted by using acid-containing methanol as solvent and patitioned with ether and chloroform. The extract of anthocyanins was purified by Amberlite XAD-7HP chro-matography. The antioxidant and free radical scavenging capacity of anthocyanin extracts were evaluated by DPPH method. The uncertainty of the antioxidant was assessed. The expanded uncertainties were 0.132 2（Trolox mg/kg）,which showed that the level of the measurement had the high quality and the data resulted by these methods was worth believing. This study will provide scientific basis for developing and utilizating of Zi juan tea and contribute the depth of processing of food materials and functional application.