补肾壮督方预防腰椎间盘退变的实验研究

An experimental study on preventing from intervertebral disc degeneration with Bushen Zhuangdu San

目的:观察补肾壮督方对大鼠腰椎间盘退变的病理及对椎间盘三磷酸腺苷(ATP)酶和肿瘤坏死因子-α(TNF-α)的影响。方法:利用针刺纤维环的方法建立大鼠椎间盘退变模型,造模成功后,随机分为空白组、模型组、补肾壮督方组、塞来昔布组,在模型建立8周后检测椎间盘组织超微量ATP酶活性和TNF-α含量,电镜扫描椎间盘胶原纤维及成纤维细胞超微结构的变化。结果:1与空白组相比,补肾壮督方组、塞来昔布组、模型组均出现明显的病理退变;与模型组相比,补肾壮督方组的病理退变较轻。2与空白组相比,模型组腰椎间盘的ATP酶活性降低,TNF-α含量升高,差异有显著的统计学意义(P<0.01);与模型组相比,补肾壮督方组腰椎间盘的ATP酶活性升高,差异有统计学意义(P<0.05),补肾壮督方组椎间盘的TNF-α含量降低,有显著的统计学差异(P<0.01)。结论:补肾壮督方具有抑制椎间盘退变作用,其机制可能与提高椎间盘组织ATP酶活性和降低TNF-α表达有关。

Objective： To investigate clinical effects of Bushen Zhuangdu San on ATP enzyme and TNF-α in rats with lumbar disc degeneration- Methods： The lumbar disc degeneration models were established by annulus puncture-The rats were divided into 4 groups randomly after successful modeling, namely： the blank control group, the model group, the Bushen Zhuangdu San group and the celecoxib capsules group- In 8 weeks, the content of ATP enzyme and TNF-α were detected; the changes of intervertebral disc collagen fibers and fibroblasts ultrastructure were scanned by electron microscopy- Results： 1 Compared with the blank control group, lumbar degeneration disces in rats in others groups were obvious- Compared with the model group, the changes of lunbar degeneration disces in the Bushen Zhuangdu San group were slighter- 2 Compared with the blank control group, the expression of ATP enzyme in the model group was reduced; the expression of TNF-α was increased in the model group; the differences were very significant（P〈0-01）- Compared with the model group, the expression of ATP enzyme was increased in the Bushen Zhuangdu San group; and the differences were very significant（P〈0-05）; the expression of TNF-α was reduced in the Bushen Zhuangdu San group; and the differences were very significant（P〈0-01）-Conclusion： Bushen Zhuangdu San can improve disc tissue ATP enzyme activity and reduce the expression of TNF-α,which may explain the mechanism of protective effect on intervertebral disc degeneration.