L1C末端共同序列多肽诱导单克隆抗体应用于宫颈脱落细胞HPV的检出

L1 C-terminal conserved sequence polypeptide monoclonal antibody in detecting human papillomavirus in cervical exfoliated cells

目的考察由人乳头瘤病毒(human papillomavirus,HPV)主要外壳蛋白(L1)C末端保守序列多肽诱导的单克隆抗体对宫颈脱落细胞内的HPV感染是否具有可应用于临床的检测能力。方法收集三峡大学第二临床医学院妇科门诊宫颈脱落细胞标本,一式两份,其中一份标本用于医院检验科HPV分型检测试剂盒检测,另一份以HPV L1C末端保守序列多肽诱导的小鼠单克隆抗体运用免疫组化方法检测标本中的HPV L1,比较两种方法检测结果的差异。结果收集到的384例妇科门诊宫颈脱落细胞标本中,HPV分型检测试剂盒检测出HPV阳性感染63例,阳性率为16.41%(63/384);用免疫组化检测了其中380例标本,共检出阳性标本120例,阳性检出率为31.58%(120/380),与HPV分型检测试剂盒检测结果相比差异有统计学意义(P<0.05)。结论初步证明了HPV L1C末端保守序列多肽诱导的小鼠单克隆抗体对宫颈脱落细胞内HPV的感染具有良好的检出能力,并且检测能力高于现临床所用的市售HPV分型检测试剂盒。该单克隆抗体在开发用于宫颈癌预防性筛查的HPV检测试剂盒方面具有一定的潜力。

Objective To observe whether the polypeptide monoclonal antibody induced by human papillomavirus（HPV）major capsid protein（L1）C-terminal conserved sequence polypeptide can be applied to detect HPV in cervical exfoliated cells.Methods Two copies of cervical exfoliated cells specimens were collected from the gynecology clinic,The Second Clinical Medicine College,China Three Gorges University：one was subjected to detection via Human Papillomavirus Genotyping Diagnosis Kit in the clinical laboratory of hospital, and the other was subjected to detection of HPV L1 via immunohistochemistry（IHC）with the mouse monoclonal antibody induced by HPV L1 C-terminal conserved sequence polypeptide.The detection results of HPV of the two methods were analyzed.Results In a total of 384 cervical exfoliated cell samples,63 were found positive for HPV by the Human Papillomavirus Genotyping Diagnosis Kit,with a positive rate of16.41%（63/384）;while IHC identified 120 positive cases,with a positive rate of 31.58%（120/380）,being significantly higher than that of the former method（P〈0.05）.Conclusion It is indicated that HPV L1 polypeptide mouse monoclonal antibody has a better detection performance for HPV infection in cervical exfoliated cells compared with Human Papillomavirus Genotyping Diagnosis Kit,and it may have a future in developing kit for the prevention screening of cervical cancer.