茜草醇提物对辐射损伤小鼠造血系统保护作用的研究

Rubiayunnanensis protects against radiation-induced hematopoietic damage in mice

目的研究茜草醇提物对辐射损伤小鼠造血系统的保护作用。方法建立辐射损伤小鼠模型,将50只小鼠随机分为5组,每组10只。给药组小鼠予以不同剂量[低剂量0.25 g/(kg·d)、中剂量0.49 g/(kg·d)、高剂量0.99 g/(kg·d)]茜草醇提物灌胃,空白对照组和单纯照射组分别予1 m L生理盐水灌胃,各组持续灌胃13 d,正常饲养7 d后处死小鼠。于灌胃第7天,不同剂量给药组和单纯照射组小鼠均接受^(60)Coγ射线6 Gy一次性全身照射。观察茜草醇提物对辐射损伤小鼠体重以及外周血白细胞、红细胞、血小板和骨髓有核细胞含量的影响。结果与单纯照射组比较,高剂量茜草醇提物给药+照射组小鼠外周血白细胞、红细胞、骨髓有核细胞计数均升高,差异均有统计学意义(P<0.05),但体重和血小板计数差异无统计学意义(P>0.05);而低剂量、中剂量茜草醇提物给药+照射组与单纯照射组比较差异均无统计学意义(P>0.05)。结论高剂量茜草提取物对辐射损伤小鼠造血系统有促进恢复作用。

Objective To explore the protective effects of rubiayunnanensis on the hematopoietic system of mice irradiated with60 Co γ-rays. Methods A mouse model of radiation damage was established and used to probe the protective effects of rubiayunnanensis. Mice were randomly divided into five groups（n =10 animals per group）：mice were given low,intermediate or high doses of an alcohol extract of madder by continuous gavage for 12 d;irradiation alone and control mice were given 1 m L normal saline. At 7 d after gavage began,all irradiation groups were irradiated with 6 Gy 60 Co γ-rays and allowed to live another 12 days,then sacrificed. Body weight;counts of erythrocytes,leukocytes,and platelets in peripheral blood;and numbers of karyota in femur were measured. Results Erythrocyte and leukocyte counts were significantly higher in animals treated with high-dose rubiayunnanensis than in irradiation alone animals（P〈0.05）,while body weight and platelet counts tended to be higher in the high-dose animals. No significant differences were observed between irradiation alone animals or animals treated with low or intermediate doses of rubiayunnanensis. Conclusions High doses of rubiayunnanensis may promote the recovery of hematological function of mice irradiated with60 Co γ-rays.