TLCS同时测定珠黄散中4种胆汁酸含量

Simultaneous Determination of Contents of Four Bile Acids in Zhuhuang San by TLCS

目的：建立同时测定珠黄散中胆酸、猪去氧胆酸、去氧胆酸、鹅去氧胆酸含量的方法。方法：以甲醇为提取溶剂,制备供试品溶液应用于薄层色谱扫描法,使用硅胶G高效薄层板,以异辛烷-乙酸乙酯-冰醋酸（15∶7∶6）为展开剂,10%硫酸乙醇溶液为显色剂,460 nm为测定波长,进行薄层定量扫描。结果：胆酸、猪去氧胆酸、去氧胆酸、鹅去氧胆酸分别在0.20~2.00μg（r≥0.999）内含量与峰面积积分值呈良好的线性关系,平均回收率（n=6）分别为95.88%（RSD=1.86%）,97.43%（RSD=2.23%）,96.24%（RSD=2.78%）,96.63%（RSD=1.87%）。测得12批珠黄散中4种胆汁酸平均含量分别为31.94,6.36,20.19,10.53 mg/g。结论：该方法专属性强,重复性好,适用于珠黄散中4种胆汁酸的含量测定及质量控制。

Objective ： To develop a method for simultaneous determination of contents of four bile acids,i.e.cholic acid,hyodeoxycholic acid,deoxycholic acid and chenodeoxycholic acid,in zhuhuang san.Methods ： Test sample solution was prepared for TLCS using methanol as an extraction solvent.Silica gel prefabricate thin layer plate was used with isooctane- ethyl acetate- glacial acetic acid（15∶7∶6） as a developing solvent and 10% alcoholic solution of sulfuric acid as a color development reagent.TLCS was performed at a detection wave-length of460 nm.Results ： There was a good linear relation between the integrated peak area and the contents of four bile acids at a range of 0.20 ~ 2.00 μg（r ≥ 0.999）.The average recovery rates（n = 6） of cholic acid,hyodeoxycholic acid,deoxycholic acid and chenodeoxycholic acid were 95.88%（RSD = 1.86%）,97.43%（RSD = 2.23%）,96.24%（RSD = 2.78%） and 96.63%（RSD = 1.87%） respectively.The average contents of the above-mentioned four bile acids in 12 batches of zhuhuang san were 31.94,6.36,20.19 and 10.53 mg/g respectively.Conclusion ： The method showed high specificity and reproducibility,which was suitable for the determination of contents of four bile acids in Zhuhuang San.