摘要
研究了越南伯克霍尔德氏菌(Burkholderia vietnamiensis)对泰乐菌素的降解效能,并通过薄层色谱法初步分离其降解产物。结果表明:用B.vietnamiensis处理初始浓度为100 mg·L^(-1)泰乐菌素药渣5 d,降解率为97.70%。用薄层色谱法初步分离的泰乐菌素降解产物,以甲醇/乙酸乙酯为展开剂,当比例调至1∶6时,可将产物初步分离为两种,降解产物A的Rf A为0.43,降解产物的Rf B为0.28;降解产物经展开后在紫外灯下可显色,说明泰乐菌素分子结构中共轭体系依然存在。
The objective of the work was to study the biodegradation capability of Burkholderia vietnamiensis to tylosin and preliminary separation of its degradation products by thin layer chromatography（TLC）. The results showed that the tested strain degraded above 97.70 % of tylosin in medium with an initial concentration of 100 mg·L^-1 after 5 d of incubation. The degradation products of tylosin are isolated with thin layer chromatography（TLC）, and the carbinol/ethyl acetate is used as developing solvent. The products can be separated into two types when the ratio is adjusted between 1∶6. The Rf A of degradation product A is 0.43 and the Rf B of degradation product B is 0.28. The degradation products are still chromogenic under ultraviolet lamp after developing. This indicates that the conjugated system still exists in the tylosin molecular structure.
出处
《广东化工》
CAS
2016年第13期32-33,24,共3页
Guangdong Chemical Industry
基金
银川能源学院科研项目(2014-KY-Y-07)
