甲基托布津对醉马草种带内生真菌的灭菌活性

Effects of thiophanate methyl on seed borne Epichloё fungal endophyte of Achnatherum inebrians

以中国西北天然草原的常见有毒植物——醉马草(Achnatherum inebrians)为研究材料,进行了内吸式杀菌剂——70%甲基托布津(thiophanate methyl)对醉马草种传内生真菌灭菌活性及种子萌发和幼苗生长影响的研究。结果表明,100倍甲基托布津可100%杀灭醉马草种传内生真菌,但该处理会显著降低(P<0.05)醉马草幼苗的发芽指数,而对其它测量指标不造成显著影响(P>0.05);其次为200倍药剂浸泡处理6 h,内生真菌杀灭率仅为61.2%,该处理种子发芽和幼苗生长与100倍药剂处理相比差异显著(P<0.05)。因此,若不考虑对种子发芽指数造成的影响,甲基托布津稀释100倍、浸种2 h为剔除醉马草种传内生真菌的最佳方法。

Achnatherum inebrians,a common toxic perennial bunchgrass in natural grassland of northwest China,was studied to explore the effect of systemic fungicide,thiophanate methyl（ Tm）,on fungal endophyte of A. inebrians at different concentrations and soak times. Tm had five concentrations and five distinct soak times respectively. The results of fungicide experiment indicated that the Tm diluted 100 times（ 100 Tm） was the most effective,but it significantly（P〈0. 05） decreased the germination index of seeds. Except the 100 Tm,the 200 times dilution Tm combined with 8 h treatment was considered to be the most effective which reduced the seeds infection rate of fungal endophyte from 100%to 38. 8%,and did not significantly（P〈0. 05） affect the growth of seedlings. In conclusion,without regard to the seed germination index,the most effective way to kill the seed borne fungal endophyte was 100 times dilution Tm combined with 2 h treatment.