微小RNA542-3p与结直肠癌细胞侵袭转移能力的相关性研究

Relationship between micro RNA542-3p and the invasion and metastasis for colorectal cancer cell

目的探讨微小RNA542-3p(miR-542-3p)在结直肠癌患者癌组织中的表达情况。方法选取40例结直肠癌患者的癌组织、配对远癌正常肠上皮组织、人正常肠上皮细胞株HIEC和结直肠癌细胞株Lovo,LS1747,SW620,HT29,SW480为研究对象。用实时荧光聚合酶链式反应法检测miR-542-3p在结直肠癌组织、正常肠上皮组织、肠癌细胞及正常肠上皮细胞株中的表达水平。通过外源转染miR-542-3p mimics或inhibitor,分别于转染后24,48,72,96 h检测HT29细胞的增殖能力;用划痕实验检测转染前后HT29细胞迁移能力评价miR-542-3p在肠癌细胞中的生物学功能。结果 MiR-542-3p表达在癌组织中显著低于正常肠上皮组织(P<0.05);miR-542-3p在结直肠癌细胞株HT29,SW620,Lovo中表达较正常肠细胞株显著降低(P<0.05),而在配对细胞株中,在高转移能力的结直肠癌细胞株SW620及Lovo中表达同样显著降低(P<0.05)。24,48,72,96 h后,miR-542-3p inhibitor组、miR-542-3p mimics组、对照组及mimics control组的细胞增殖能力比较差异无统计学意义(P>0.05)。24 h后,miR-542-3p mimics组迁移能力显著低于mimics control组(P<0.05),而miR-542-3p inhibitor组迁移能力显著高于inhibitor control组(P<0.05)。结论 MiR-542-3p与结直肠癌细胞的侵袭转移能力有关,可作为肠癌侵袭转移的分子标志物。

Objective To evaluate the molecular mechanism of micro RNA542- 3p（ miR- 542- 3p） in the invasion and metastasis of colorectal cancer. Methods Forty paired colorectal cancer patients with cancer tissue and relative normal colorectal tissue,different metastasis ability colorectal cancer cell lines（ Lovo,LS1747,SW620,HT29,SW480）and normal colorectal epithelial cells were tested of the miR- 542- 3p expression by real- time polymerase chain reaction. The ability of proliferation or migration for SW480 or HT29 cells were tested after transinfection of miR- 542- 3p,mimics and inhibitor after 24 h. The physiological characteristics such as proiferation,migration of colon cancer cells was evaluated. The biological function of miR- 542- 3p was evaluated in colorectal cancer cells. Results MiR- 542- 3p expression in cancer tissues was significantly lower than that in normal intestinal epithelium（ P〈0. 05）. MiR- 542- 3p was higher expressed in normal colorectalepithelial cells compared to colorectal cancer cell lines（ HT29,SW620 and Lovo,P〈0. 05）. MiR- 542- 3p was higher expressed in the high metastasis cancer cell lines（ SW620 and Lovo） compared to low metastasis cancer cells（ P〈0. 05）. After 24,48,72 and 96 h,the cell proliferation ability was no significant difference in inhibitor mir- 542- 3p inhibitor group,miR- 542- 3p mimics group,control group,mimics control group（ P〈0. 05）. After24 h,the migration ability in mir- 542- 3p mimics group was significantly lower than that of mimics control group（ P〈0. 05）,and the migration ability in miR- 542- 3p inhibitor group was significantly higher than that of inhibitor control group（ P〈0. 05）. Conclusion MiR- 542- 3p may play a role in inhibiting cell metastasis in colon cancer cells,which can be used as biomarker for colorectal cancer metastasis.