硫化氢对肾血管性高血压大鼠动脉舒张功能的调节作用

Effects of hydrogen sulfide on arterial vasodilatation function of renal hypertensive rats

目的探讨硫化氢(H2S)对肾血管性高血压模型大鼠血压及颈总动脉血管舒张功能的影响。方法采用"两肾一夹(2K1C)"法建立肾血管性高血压模型。大鼠随机分为假手术组、双肾一夹(2K1C)模型组、2K1C+Na HS(H2S供体)组及PPG(H2S代谢酶抑制剂)组。术前及术后每周测量1次大鼠尾动脉收缩压(SBP)。测定颈总动脉血管形态学指标(血管外周、壁厚、壁厚与外周径的比值),观察颈总动脉的ACh介导的内皮依赖性舒张作用;免疫组化检测颈总动脉一氧化氮合成酶(e NOS)、内皮素-1(ET-1)的表达。结果PPG组及2K1C组大鼠尾动脉血压与sham组比较明显升高(P<0.05);与模型组比较,2K1C+Na HS组血压明显降低,大鼠颈总动脉壁厚外径比减小,颈总动脉对ACh介导的内皮依赖性舒张效应增强,颈总动脉一氧化氮合成酶(e NOS)含量升高、ET-1含量降低。结论给予外源性的硫化氢供体可降低血压,缓解肾血管性高血压的形成,其作用可能与改善颈总动脉血管功能有关。

Aim To investigate the modulation effects of NaHS on arterial vasodilatation functions of renal hy-pertensive rats .Methods Two-kidney , one-c lip （ 2K1C ） renovascular hypertension was induced .Rats were randomly divided into four group：sham group , two-kidney one-clip model （ 2K1C ） group, 2KIC ＋NaSH（ H2 S donor ） group, PPG group.The systolic blood pressure （ SBP ） was measured before the opera-tion and each week after the operation .The carotid ar-tery was collected for morphometric parameters （ outer radius, wall thickness, the radio of wall thickness to outer radius） and the tension of the carotid artery was observed with the isolated artery ring technique .Immu-nohistochemistry was used to determine the protein ex-pression of eNOS , ET-1 protein in carotid artery .Re-sults The blood pressure of 2K1C group and PPG＆nbsp;group was higher than that of sham group （ P〈0.05） . Compared with 2K1C group,the blood pressure and the rat arteria carotis communis of the radio of wall thick-ness to outer radius of 2K1C＋NaHS group decreased significantly , while the relaxation of carotid artery to ACh in NaHS group increased .According to the immu-nohistochemistry results , eNOS expression was upregu-lated while ET-1 was downregulated in 2K1C ＋NaHS group as compared with 2K1C group.Conclusions Chronical administration of NaHS can decrease blood pressure in renovasocular hypertensive rats .The anti-hypertensive effect of H 2 S maybe associated with im-provement of the arterial functions .