P16、Ki-67在宫颈鳞状上皮内病变中的表达及其意义

Study on the expression and diagnostic value of Ki67,P16 in cervical squamous intraepithelial lesion

目的:研究P16、Ki-67在萎缩性宫颈、鳞状上皮化生及宫颈鳞状上皮内病变(SIL)中的表达情况,探讨16、Ki-67在鉴别上述病变中的应用。方法:选取50例萎缩性宫颈、50例鳞状上皮化生及150例SIL,其中低级别鳞状上皮内病变(LSIL)病变50例,高级别鳞状上皮内病变(HSIL)病变100例。应用免疫组化SP法检测P16及Ki-67在上述组织中的表达,统计其阳性率及表达模式,分析其在鉴别上述病变中的意义。结果:P16、Ki-67在萎缩性宫颈、鳞状上皮化生仅在基底层及副基底层可见少量细胞阳性表达,P16阳性率分别为:10%、14%;Ki67阳性率分别为:14%、16%。P16、Ki-67在SIL中呈阳性表达,阳性率分别为:86.7%、88.0%。与萎缩性宫颈、鳞状上皮化生的阳性率比较,差异有显著统计学意义(P<0.05)。在表达模式上,LSIL中P16、Ki-67阳性细胞散在分布于上皮下1/3,表层一般无阳性表达的细胞。HSIL中P16、Ki-67阳性细胞较多,阳性细胞可分布于上皮表层,乃至上及全层。结论:P16、Ki-67可协助鉴别萎缩性宫颈、鳞状上皮化生及SIL,而且其表达模式能协助鉴别LSIL与HSIL。

Objective To study the expression of P16 and Ki -67 in cervical squamous intraepithelial lesion and to explore the diagnostic value of them.Method The expressions of P16 and Ki67 were detected by immunohistochemical SP method in 50 cases of atrophica cervix,50 cases of metaplastic cervix and 150 cases of cervical squamous intraepithelial lesion.Results The negative and sporadic expression of Pl6 and Ki -67 were observed in atrophica cervix and metaplastic cervix,the positive rates of P16 were 10% and 14%,respectively,the positive rates of Ki67 were 14% and 16%,respectively.The positive and undispersed expression of P16 and Ki -67 were observed in cervical squamous intraepithelial lesion,the positive rates were 10% and 14%,respective-ly.There were singificant difference betweem SIL and others.The positive cells of P16 and Ki67 were distributed sporadically in the bottom of the epithelium in LSIL,and were distributed intensively in the superior of the epithelium in HSIL.Conclusion P16 and Ki -67 have certain auxiliary effect on the diagnose and classify of cervical squamous intraepithelial lesion.