贴梗海棠花粉生活力的测定

Studies on Measurement Methods of Pollen Viability

以贴梗海棠初开花朵为试验材料,研究了TTC、碘-碘化钾2种染色剂测定花粉生活力的效果,离体培养时葡萄糖对花粉萌发的影响和3种不同温度(15、20、25℃)条件下的花粉萌发的变化规律,3种贮藏条件(常温、4℃、-23℃)对花粉贮藏的影响。结果表明:不同染色剂测定的花粉生活力差异显著,TTC染色法(76.26%)显著优于碘-碘化钾染色法(42.46%)。在一定范围内,培养基葡萄糖浓度和萌发温度提高能促进花粉的萌发,超出范围后抑制萌发。试验证明:最佳的葡萄糖浓度为10%,最佳的萌发温度为20℃。贮藏温度对花粉生活力的影响显著,其中最佳的贮藏条件是冷冻干燥,但综合考虑成本、便捷性等因素,实际应用中一般选择4℃。

The birth of quince flowers as experimental material,studied the TTC,iodine,potassium iodide determination of two kinds of stain pollen viability,the effect of glucose on pollen germination in vitro culture and the influence of three different temperature）15℃。20℃,25℃）under the condition of the rule of variation of pollen germination,three kinds of storage conditions（temperature,4℃,-23℃）affect pollen storage.The results showed that：significant difference was found in determination of different stain of pollen viability,TTC staining（76.26%）were significantly better than iodine-potassium iodide staining（42.46%）.Within a certain range,glucose and temperature can promote the germination of pollen,beyond the scope of inhibiting germination,the experiment proved that the best glucose concentration was 10%,the best germination temperature is20℃.Storage temperature has significant influence on pollen viability,one of the best storage conditions is 4℃.