牙囊干细胞成骨分化基因调控网络的生物信息学分析

Gene regulatory network during the osteogenic differentiation process of dental follicle stem cells: A bioinformatic analysis

目的:应用生物信息学分析牙囊干细胞(DFSCs)成骨分化过程中基因调控网络。方法:从GEO Datasets数据库获取在DFSCs成骨分化过程中差异表达的基因。应用DAVID和Gene MANIA数据库对这些基因的关系进行分析。结果:从GEO Datasets数据库获得了大量DFSCs成骨分化过程中差异表达的基因,对差异表达明显(P<0.05)的289个基因进行分析,富集到"细胞分化"、"细胞增殖"、"骨骼系统发育"、"钙信号通路"等基因子集。其中ALPL、CTSK、TUFT1、TGFBR2、FHL2、ROR2、STC1、POSTN,ZBTB16、FRZB、PRELP、IGFBP5等12个基因被富集在"骨骼系统发育"子集,这12个基因通过共表达、共定位、遗传学相互作用等彼此关联并与其它信号通路相联系。结论:DFSCs成骨分化中许多差异表达的基因间存在相互作用的分子网路。需要从WNT、TGFbeta、MAPK等信号通路、同源盒转录因子及成骨分化标记物基因的整体关系上来认识DFSCs成骨分化。

Objective： To study the gene regulatory network during the osteogenic differentiation process of dental follicle stem cells（ DFSCs） with bioinformatic analysis. Methods： The differentially expressed genes during the osteogenic diffrentiation process of DFSCs were selected from the GEO Datasets. Then the relationship among the genes were analysed using DAVID and Gene MANIA database. Results： Numerous differentially expressed genes during the osteogenic differentiation process of DFSCs were obtained,289 genes with significance of different expression（ P〈0. 05） were enriched into＂cell differentiation＂,＂cell proliferation＂,＂skeletal system development＂and ＂calcium signaling pathway＂subgroups. ALPL,CTSK,TUFT1,TGFBR2,FHL2,ROR2,STC1,POSTN,ZBTB16,FRZB,PRELP and IGFBP5 were enriched into the＂skeletal system development＂subgroup. The 12 genes exhibited interactions,including co-expression,co-localization,genetic interaction and signal pathways. Conclusion： There is a complex molecular regulatory network among the differentially expressed genes during the osteogenic differentiation process of DFSCs. It is necessary to analyse the osteogenic differentiation process of DFSCs as a whole from WNT,TGFbeta,MAPK siganal pathyways,Homeobox transcription factors and osteogenic differentiation marker genes.