基于表型与分子数据的斑茅核心种质构建

Constructing Core Collection of Saccharum arundinaceum L. Based on Phenotype and Molecular Markers

斑茅(Saccharum arundinaceum L.)是重要的"甘蔗复合群"野生资源,在我国分布广泛,但目前尚未在遗传育种研究中得到很好的利用。本研究以国家甘蔗种质资源圃保存的来自全国9省区的147份斑茅无性系为原始种质,首先基于表型数据筛选58份材料构建了斑茅初级核心种质,然后基于SSR和AFLP分子标记数据筛选16份材料构建斑茅微核心种质,分别占原始种质的39.46%和10.88%。通过表型与分子遗传多样性参数的比较分析,验证了斑茅核心种质具有较好代表性。最后对甘蔗种质资源和遗传育种研究中斑茅核心种质的构建方法和利用策略等问题进行了探讨,以期为"甘蔗复合群"野生类群核心种质的构建及创新利用提供参考,促进资源研究工作重心由数量保存型向创新利用型转变。

As one of the important wild germplasm resources in Saccharum complex,Saccharum arundinaceum L. widely distributed in China,but has being used inefficiently in sugarcane genetic breeding research. In this study,we gathered 147 clones of S. arundinaceum L. from 9 provinces in China and planted in National Germplasm Repository of Sugarcane as original materials. Firstly,58 clones were selected based on phenotypic data to construct primary core collection and then 16 clones were selected based on SSR and AFLP molecular markers to construct mini core collection,which occupied 39. 46% and 10. 88% of original materials,respectively. Through comparative analysis of phenotypic and molecular genetic diversity parameters,the core collection of S. arundinaceum L. was proved to be a good representative sample. The construction approach and effective utilization of the core collection of S. arundinaceum L. in sugarcane germplasm resources and genetic breeding research were discussed. This paper provides a reference for construction and application of core collection from wild populations of Saccharum complex,and promotes change of the future research from quantitative conservation to innovative utilization.