摘要
目的:探讨加味七方胃痛颗粒对胃癌癌前病变(precancerous lesions of gastric cancer,PLGC)大鼠CDK2/4基因的干预效应。方法:采用化学致癌物N-甲基-N'-硝基-N-亚硝基胍(N-methyl-N-nitro-N-nitroso-guanidine,MNNG)建立胃癌癌前病变动物模型,采用免疫组化检测CDK2/4基因表达,Western blot法检测各组大鼠PLGC胃黏膜组织CDK2/4的表达水平。结果:与模型组比较,加味七方胃痛颗粒组和胃复春组的胃黏膜萎缩程度、胃黏膜肠化生程度明显减轻;加味七方胃痛颗粒组、胃复春组CDK2/4基因蛋白表达显著降低(P<0.01);相对于胃复春组,加味七方胃痛颗粒CDK2/4蛋白表达显著降低(P<0.01)。结论:加味七方胃痛颗粒可能是通过下调CDK2/4基因蛋白的表达,改善胃黏膜病理变化,抑制细胞分裂与增殖,从而干预PLGC进展。
Objective: To observe the effect of Modified Qifang Weitong Granule on CDK2 /4 genes in treating for rat's precancerous lesions of gastric cancer. Methods: animal model of precancerous lesions was first established by using N-methyl-N-nitro-N-nitrosoguanidine( MNNG). CDK2 /4 gene expressions were detected with immunohistochemistry method. The expression levels of CDK2 /4 in rats' PLGC gastric tissue were detected with Western blot method. Results: Compared with those of the model group,the degrees of gastric atrophy and gastric intestinal metaplasia significantly relieved and the CDK2 /4 gene and protein expression was significantly lower( P 〈0. 01) of Qifang Weitong Granule group and Weifuchun group. Compared with that of the Weifuchun group,the CDK2 /4 protein expression of Qifang Weitong Granule group was significantly lower( P 〈0. 01). Conclusion: Modified Qifang Weitong Granule can achieve better clinical efficacy by reducing CDK2 /4 gene and protein expressions,improving the conditions of gastric mucosa,inhibiting cell division and proliferation,and eventually preventing the progress PLGC.
出处
《中医学报》
CAS
2016年第7期928-930,共3页
Acta Chinese Medicine
基金
国家自然科学基金资助项目(81260535)
广西硕士研究生科研创新项目(YCSZ2014173)
关键词
胃癌癌前病变
加味七方胃痛颗粒
胃复春
CDK2/4
大鼠
precancerous lesions of gastric cancer
Modified Qifang Weitong Granule
Weifuchun
CDK2 /4
rat