染料木素联合多不饱和脂肪酸对MCF-7细胞脂代谢基因表达的影响

Effects of Genistein Combined Polyunsaturated Fatty Acids on Lipid Metabolic Gene Expression of MCF-7 Cells

选用不同浓度的染料木素(GEN)及二十二碳六烯酸(DHA)作用于MCF-7细胞,研究大豆中抗癌成分染料木素与不同比例的多不饱和脂肪酸(PUFAs)联用,对乳腺癌细胞MCF-7增殖及脂代谢基因表达情况的影响。根据DHA的有效浓度设定亚油酸(LA)及LA∶DHA(10∶1)混合PUFAs作用浓度。将GEN分别与DHA、LA及LA∶DHA(10∶1)联合作用细胞,检测细胞生存率,并通过qPCR方法检测细胞内过氧化物酶体增殖物激活受体γ(PPARγ)、5-脂氧合酶(5-LOX)、环氧合酶-2(COX-2)mRNA表达情况。结果表明:高浓度GEN(300,200,100μmol·L^(-1))及DHA(400,300,200μmol·L^(-1))可抑制MCF-7细胞的增殖,且随着时间增加抑制作用增强。而低浓度GEN(50,25μmol·L^(-1))及LA组及10∶1组对细胞增殖有轻度促进作用。添加GEN的G+L组和G+10∶1组细胞的生存率显著降低,同时GEN可降低G+L组细胞5-LOX mRNA的表达量,且GEN与LA∶DHA(10∶1)联用,增加了细胞内PPARγ mRNA的表达水平并抑制了COX-2 mRNA的表达。试验证实GEN可抑制高比例ω-6 PUFAs对乳腺癌细胞增殖的促进作用,其作用机制主要是通过增加PPARγ mRNA表达,抑制COX-2 mRNA表达水平实现的。大豆的抗乳腺癌作用可能是由于GEN与PUFAs在抑制乳腺癌细胞增殖方面产生了协同效应。

The effect of gentistein which is the anticancer component in soy, combined different ratios polyunsaturated fatty acids （PUFAs） on proliferation and lipid metabolic gene expression of MCF-7 cells was studied. MCF-7 was cultured with different concentration gentistein（GEN） and docosahexaenoic acid（DHA）. According to the effect concentration of DHA, we set linoleic acid（LA） and mixed PUFAs＇ concentration, which was 10：1 LA to DHA. Then we combined GEN with DHA, LA and LA： DHA （ 10：1 ） respectively, texted the survival rate and detect mRNA expression of peroxisome proliferators-activated receptorγ（ PPARγ）, 5-1ipoxygenase （5-LOX） and cyclooxygenase-2 （COX-2） with qPCR. The results showed that high concentration of GEN and DHA could inhibit proliferation of MCF-7 cells and the effect enhanced with time. However, low concentration of GEN could promote the cells proliferation mildly. LA and 10：1 PUFAs promote the cells proliferation, but cell＇s proliferation was inhibited when we added GEN. GEN could reduce 5-LOX mRNA level of MCF-7 cell which was under the effect of LA. Meanwhile it inhibits proliferation of MCF-7 cell through promoting PPARγ mRNA level and inhibiting COX- 2 mRNA level when the cell is cultured with GEN combined 10：1 LA： DHA. GEN can inhibit the promoting effect of high ratio ω-6 PUFAs＇ proliferation of breast cancer cells. The mechanism is mainly through improving the expression of PPART mRNA and inhibiting COX-2 mRNA. The synergistic effect of GEN and PUFAs on inhibiting the proliferation of breast cancer cells, may be the reason for the anti breast cancer of soy.