摘要
以大豆球蛋白特异性多克隆抗体为一抗,辣根过氧化酶标记抗兔IgG(HPR)为酶标二抗,TMB为底物,建立一种检测大豆球蛋白直接ELISA方法。应用该方法对在吉林省部分地区收集的25个样本进行初步检测,并与竞争ELISA方法进行对比,每个样品进行6个重复,结果显示,约64%的样本使用直接ELISA法的蛋白检出显著高于竞争ELISA法,且标准差的符合率达到72%以上。本试验建立的直接ELISA方法具有较高的敏感性和特异性,适于食品及饲料行业中过敏蛋白的批量检测。
The present study presents a direct enzyme-linked immunosorbent assay （ELISA） that allows for the detection of trace amount of glycinin in soybean. In this assay, rabbit anti-glycinin polyclonal antibody（Pab） was used as primary antibody and goat anti-mouse igG-horseradish peroxidae（HRP） was used as secondary antibody. Twenty-five samples collected in parts of Jilin province were preliminary tested by this detection method and compared with competitive ELISA method. Results showed amounts of glycinin in about 64% samples detected by the dcELISA were significantly higher than that detected by competitive ELISA and the coincidence rate of the standard deviation（SD） was more than 72%. The method showed a good sensitivity and specificity and was suitable for quick and large quantitative detection of glycinin in food and feed industry.
出处
《大豆科学》
CAS
CSCD
北大核心
2016年第4期660-665,共6页
Soybean Science
基金
国家自然科学基金青年基金(31572439)
吉林省自然科学基金(20160101348JC)
国家"十二五"科技支撑计划(2013BAD17B0)
教育厅"十二五"科学技术研究资助项目(2015198)
