大豆花叶病毒病种粒斑驳抗性基因定位

Mapping of Gene with Resistance to Seed Coat Mottle in Soybean

大豆种粒斑驳严重影响大豆商品性,选育抗病品种可有效控制大豆种粒斑驳。利用抗源东农93-046与品1246所衍生的F_(8∶9)代群体,对其进行种粒斑驳鉴定,同时利用分子标记对其抗性基因进行初步分析,结果表明:以斑驳率5%为界限划分抗感株系,F_(8∶9)代抗病株系数与感病株系数基本符合1∶1的比例,这表明东农93-046的种粒斑驳抗性受一对等位基因控制。根据前人构建的一个包括13个分子标记F连锁群的遗传连锁图谱,结合单标记和复合区间作图法将东农93-046抗性基因位点定位于SSR分子标记Satt114附近。

Soybean seed coat mottle seriously affects on soybean commodity. The selection of resistant varieties could efficiently control this disease. The aim of this study is to map the resistant gene of resisance cultivar Dongnong 93-046 through a F8：9 populations from a cross between Dongnong 93-046 and Pin 1246. The results showed the ratio （resistance line number： susceptible line number） was 1：1 in the F8：9 generation, which suggested that the resistance of Donong 93-046 was controlled by a pair of alleles. A total of 13 SSR markers in linkage group F were used to construct a genetic map. Resistance gene of Dongnong 93-046 was located near the SSR marker Satt114 through single marker analysis and composite interval analysis. It offered a good foundation for molecular marker assisted selection.