不同处理对三种药用植物外植体生长发育的影响

Effect of Different Treatments on the Growth and Development of Three Kinds of Explants of Medicinal Plants

以红豆杉、无花果、大叶黄杨的植物茎段为外植体,研究了不同浓度琼脂与不同灭菌时间处理对3种药用植物外植体褐化、污染及发芽情况的影响。结果表明：随着琼脂浓度的升高,褐化率、褐化等级、污染率和发芽率均呈下降趋势;综合各项指标分析,以琼脂浓度10g·L-1效果较好。随灭菌时间增加,褐化率、褐化等级上升,但污染率、发芽率均急剧下降,综合各项指标分析,以灭菌时间5~10min的处理效果较好。

Taking Taxus chinensis ,Ficus carica L. and Euonymus japonzcus Thumb. as materials, the effects of different agar concentration and different sterilization time on browning, pollution and germination were studied. The results showed that with the increase of agar concentration, the browning rate, browning degree, pollution rate and germination rate were all decreased. Comprehensive analysis of indicators, the effect of the concentration of agar 10 g ·L-1 was better. With the increase of sterilization time, the browning rate and browning degree increased, but the pollution rate and germination rate decreased sharply. The treatment effect of the sterilization time 5--10 minutes was better.