农药百菌清对核桃黑斑病伴生病原菌成团泛菌的抑菌活性研究

Antibacterial Activity Evaluation of Chlorothalonil in vitro Against Pantoea agglomerans on Walnut

以核桃黑斑病伴生病原菌成团泛菌(Pantoea agglomerans)为供试菌,采用抑菌圈法和平板菌落计数法,研究了农药百菌清对核桃黑斑病伴生病原菌成团泛菌(Pantoea agglomerans)的抑菌作用。结果表明:农药百菌清对该病原菌具有较好的抑菌作用,50.00mg·mL-1的农药百菌清处理96h后,抑菌圈的平均直径为1.80cm,其抑菌率达到93.9%,且具有时间与剂量效应。2种试验方法的抑菌趋势是一致的,平板菌落计数法测定的结果变化趋势较抑菌圈法明显。

Taking Pantoea agglomerans as test bacteria, the method of bacteriostatic circle and plate culture count were used to study chlorothalonil control effect on bacteriostasis of associated pathogen （Pantoea agglomerans）. The results showed that chlorothalonil had good effect on bacteriostasis. Treatment with the concentration of 50. 00 mg·mL-1 chlorothalonil for 96 hours,antibacterial rate reached to 93. 9%, in a dose and time dependent manner. The trends of antibacterial by two test methods were the same,but the results of plate culture count were more obvious than those of method of bacteriostatic circle.