多汁乳菇多糖的微波辅助提取工艺及其清除DPPH自由基研究

Microwave-assisted Extraction and Antioxidant Activity of Polysaccharides From Lactarius volemus Fr.

以多汁乳菇为试材,研究了液料比、微波功率、提取时间、提取次数对多糖提取率的影响,并采用正交实验优化了工艺条件,对提取产物进行了DPPH自由基清除研究。结果表明:对多糖提取率影响最大是微波功率,其次是液料比,提取时间影响最小;优化工艺条件为液料比20∶1mL·g^(-1),微波功率480W,提取时间4min,提取3次。在此条件下多糖提取率达6.41%。多糖对DPPH自由基有一定的清除作用,证明多汁乳菇多糖具有一定的抗氧化活性。

Taking Lactarius volemus Fr. as the raw material,the effects of extraction conditions such as ratio of liquid-solid, microwave time,microwave power and time of extraction were investigated by one-factor tests and orthogonal test,and the extraction polysaccharides analyzed with DPPH· test. The results showed that the microwave power affected the yield markably,the second was ratio of liquid-solid, and microwave time had the least influence; the optimum coditions were determined as follows：ratio of liquid-solid of 20 ：1 mL· g-1 ,microwave power of 480 W,microwave time of 4 min and times of extraction of 3 times. Under the condition,the yield was up to 6. 41%. Polysaccharides exhibited antioxidant effects in 2,2t-diphenyl-l-picrylhydrazyl（DPPH· ） radical scavenging assay. And it showed that the extraction polysaccharides which had some oxidation resistance.