Th-POK及其相关因子在胃癌组织中的表达及临床意义

Expression and clinical significance of Th-POK and related factors in gastric cancer tissues

目的探讨胃癌组织中Th-POK、CD4、CD8、Th-POK相关因子RUNX3和颗粒酶B(GZMB)的表达及临床意义。方法收集2011年3月至2014年10月100例胃癌手术组织及配对癌旁组织标本。采用免疫组化PV-6000二步法检测胃癌及癌旁组织中Th-POK、CD4、CD8、RUNX-3和GZMB蛋白的表达情况。分析Th-POK及其相关因子与胃癌临床病理特征的关系。结果 Th-POK和CD4在胃癌组织中的阳性表达率均高于癌旁组织(80%vs.67%,P<0.05;77%vs.60%,P<0.05),CD8和RUNX-3在癌旁组织中阳性表达率显著高于胃癌组织(80%vs.60%,P<0.01;86%vs.59%,P<0.01)。GZMB在胃癌组织中的阳性表达率高于癌旁组织,但差异无统计学意义(70%vs.61%,P>0.05)。Th-POK和RUNX3表达均与TNM分期有关(P<0.05),与年龄、性别、部位、分化程度及淋巴结转移无关(P>0.05)。Th-POK表达与CD4表达呈正相关(r=0.200,P<0.05),与RUNX3表达呈负相关(r=-0.373,P<0.001),与CD8及GZMB表达均无关(P>0.05)。结论胃癌组织Th-POK表达增强,其可能通过抑制RUNX3表达、减少CD8表达、促进CD4表达,从而促进胃癌的免疫逃逸。

Objective To investigate the expression and clinical significance of T-helper-inducing POZ/Krtippel-like factor （Th-POK） CD 4, CD 8, and its related factors RUNX3 and granzyme B （GZMB） in gastric cancer. Methods From March 2011 to Oc- tober 2014, 100 gastric cancer tissues and adjacent normal tissues were collected. Immunohistochemical PV-6000 staining was used to detect Th-POK expression in gastric cancer tissue and normal adjacent tissues, as well as the expression of CD4, CD8, RUNX3 and GZMB protein. The correlation of their expression with clinicopathological features was analyzed. Results The positive expression of Th-POK and CD4 were higher in gastric cancer tissues than those in adjacent normal tissues（ 80% vs. 67~/0, P〈0. 05; 77% vs. 60%, P 〈0. 05 ）. The positive expression of CD8 and RUNX-3 were lower in gastric cancer tissues than those in adjacent normal tissues （80% vs. 60%, P〈0. 01 ; 59% vs. 86%, P〈0. 01 ）. There was no significant difference of GZMB positive expression rate between gastric cancer tissues and adjacent normal tissue（ 70% vs. 61%, P〉0. 05）. The expression of Th-POK and RUNX3 were correlated with TNM stage （ P〈0. 05 ） , but not with age, tumor site, differentiation and lymph node metastasis （ P〉0. 05）. Th-POK was positively correlated with CD4 （r = 0. 200, P〈0. 05 ） , and negatively correlated with RUNX3 （r =-0. 373, P〈0. 001 ）.There was no significant correlation be- tween expression of Th-POK and GZMB or CD8 （P〉0. 05）. Conclusion The expression of Th-POK in gastric cancer tissue is up-reg- ulated, which may interact with RUNX3 to counteract the generation of CD8＋ lymphocytes, increases the generation of CD4＋ lympho- cytes and assists gastric cancer immune escape.